人参三醇皂甙促进人白细胞介素6转译的生物学效应

Effect of panaxatriol ginsenoside on interleukine-6 mRNA translation

将PHA激活淋巴结细胞作为研究对象,观察了人参三醇皂甙(PTGS)对人白细胞介素6(IL-6)分泌的促进效应。应用同位素示踪技术观察细胞RNA合成和蛋白质合成与IL-6分泌的关系,结合IL-6mRNA体外转译体系,探讨了PTGS促IL-6诱生的机理。结果表明PTGS对IL-6基因的转录环节无明显作用,但对IL-6基因的转译环节具明显促进效应。

PTGS (panaxatriol ginsenoside) and TPA (tetradecanoyl phorbol acetate) could promote the protein synthesis ( [3H] leucine uptake) by 55% and 65% respectively, and then increase the interleukine-6 (IL-6) production (biological activity) by 100% and 210% respectively, on PHA-activated human lymph node cells. TPA could promote the RNA synthesis ( [3H] UR uptake) by 80%, which is well-known to relate to up-regulatory effect of TPA on gene transcription of IL-2, but PTGS could not promote the RNA synthesis. It is supposed that PTGS promotes the IL-6 production through up-regulation of IL-6 mRNA translation. Furthermore, it was observed that IL-6 mRNA from PHA+PTGS group translated more IL-6 (4.20 U/μg RNA) than PHA group (1.35 U/μg RNA) and PHA+TPA group (1.60U /μg RNA) in wheat germ cell-free system (WGS). The translational efficiency of PHA+PTGS group (6.39%) was higher than PHA group (4.73%) and PHA+TPA group (3.14%) if the IL-6 translated in WGS was compared with IL-6 secreted in cell culture of same amount of cells. Finally, PTGS was observed to promote invitro translation of IL-6 mRNA dose-dependently (maximally by 135%) when added directly to WGS, in order to simulate the effect of PTGS within lymph node cells. It is suggested that PTGS might make PHA-induced IL-6 mRNA more effective for translation.