基于UHPLC-QTOF/MS的博落回花中生物碱类化学成分研究

Identification of Alkaloids in Flowers of Macleaya cordata(Willd) R. Br. by UHPLC-QTOF/MS

目的:利用高通量、高灵敏和快速的UHPLC-QTOF/MS技术开展博落回花中的微量生物碱类成分的定性研究,阐明博落回花中生物碱类化学成分的类别和种类,为博落回植物的安全性提供科学依据。方法:采用UHPLC-QTOF/MS技术,借助数据挖掘方法发现和识别出博落回花中的微量生物碱类化学成分,对其高分辨一级和二级质谱数据进行解析,并与数据库进行比对,完成对微量生物碱类化学成分的结构推测和确认。结果:从博落回花中共鉴定了22个生物碱类化合物,主要化学成分为普洛托品类生物碱(原阿片碱、别隐品碱)和四氢原小檗碱类生物碱(N-甲基刺罂粟碱、N-甲基四氢小檗碱)。结论:该研究结果为博落回植物的更全面、客观的品质评价和安全性评价提供了充分的科学依据。

Objective： To elucidate the minor alkaloids in the flower of Macleaya cordata by using the uhra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry （UHPLC-Q-TOF/MS） for safety evaluation of M. cordata. Methods： The alkaloids were found and identified through the serious data mining, structural speculation on basis of the careful analysis of the MS and MS/MS data, and confirmed by comparison with the reference standards and library. Results ： There were total 22 alkaloids found in the flower of M. cordata including protopines and tetrahydroprotonerberines, the main alkaloids including protopine, allocryptopine, N-methyltetrahydroberberine, N-methyhetrahydropapaverine. Conclusion ： The results provide sufficient scientific evidence for evaluating its quality and safety of the flowers from M. cordata comprehensively and objectively.