污染河流土著异养硝化菌的筛选及其鉴定

Isolation and Identification of Indigenous Heterotrophic Denitrifying Bacteria from Polluted River

利用以琥珀酸钠和硫酸铵为唯一碳源和氮源的选择培养基从贾鲁河污染水体中筛选异养硝化菌,采用富集、梯度稀释涂布平板和平板划线分离的方法对菌种进行分离纯化,结合16S r DNA分析、生理生化特性和氮转化特点对菌种进行鉴定。结果表明,从水体中共分离出的63株纯菌株中,经鉴定其中3株菌为异养硝化菌,包括1株硝化假单胞杆菌(Pseudomonas nitroreducens)和2株门多萨假单胞杆菌(Pseudomonas mendocina),对氨氮去除率分别为91.8%、89.8%、81.4%。

Heterotrophic denitrifying bacteria from the polluted water of Jialu River were screened on the selective medium,withsodium succinate as sole carbon source and ammonium sulfate as sole nitrogen source. Then,the separation and purification were performedby enrichment,gradient dilution and streaking on the plates. Finally,the strain was identified according to 16 S r DNA sequence alignmentresults,physiological and biochemical experiments and the ammonia nitrogen transformation. The results showed that,63 strains were isolatedfrom the polluted water,and 3 of them were identified as heterotrophic denitrifying bacterium,including a strain of Pseudomonas nirtoreducensand two strains of Pseudomonas mendocina,by which the ammonia nitrogen removal rate were 91.8%,89.8%,and 81.4%,respectively.