氨基嘧啶对脊髓腹角神经细胞生长的影响

Effect of aminopyrimidine on growth of ventral spinal cord cells

本文观察了氨基嘧啶对鼠胚脊髓腹角神经细胞生长的作用。不同浓度的氨基嘧啶使长突起的细胞数及突起数增加,有明显量效关系;氨基嘧啶能对抗秋水仙素对神经细胞突起的生长和提取的兔脑微管亚基管蛋白体外聚合成微管的抑制作用,又能使蛋白质含量高的细胞数增多。上述结果提示氨基嘧啶可能通过保护管蛋白聚合和增加蛋白合成而促进神经细胞的生长。

We have studied the effect of aminopyrimidine on growth of rat spinal cord ventral cells in culture. The number of cells with which projected processes and the number of neurites were increased by aminopyrimidine under a significant dose-effect relationship. Aminopyrimidine also abolished the inhibitory effect of colchicine on cell growth. Further, we purified tubulin, the subunit of microtubule from rabbit brain and aminopyrimidine could decrease the inhibitory effect of colchicine on tubulin assembly. The result of flow cytometry showedthat the number of cell with high protein content were increased by aminopyrimidine. These results suggested that the anti-colchicine effect of aminopyrimidine on cell growth could be concerned with the protection of aminopyrimidine on microtubule assembly and that the promotive effect on cell growth of amino-pyrimidine could by concerned with the protein synthesis of cultured cells.