异常胆液质证大鼠尿液代谢组分的变化及其意义

Metabonomic analysis of the urine from rat model with abnormal sapra syndrome

目的:基于代谢组学方法研究异常胆液质证大鼠尿液中的差异性代谢组分,并通过差异性代谢组分结果探讨异常胆液质证的分子基础。方法:选用Wistar大鼠,通过干热环境、干热性饲料、慢性电刺激等多复合因素建立维吾尔医学异常胆液质载体动物模型,通过代谢组学方法检测尿液中的差异性代谢物。结果:异常胆液质证大鼠尿液具有显著性高峰度代谢物为异亮氨酸、亮氨酸、糖蛋白,具有显著性低峰度代谢物为谷氨酰胺、肌酸、柠檬酸、苯丙氨酸。结论:多因素复合刺激可促进异常胆液质的产生,异常胆液质证大鼠存在三大营养物质及能量代谢障碍,可能是异常胆液质证的分子基础。

Objective： To investigate the correlation between the change in metabolic components of urine and the abnormal sapra syndrome by using a rat model of abnormal sapra syndrome. Methods： Multiple factors, such as dry environment, dry feed, and chronic electrical stimulation, were used to establish the abnormal sapra syndrome in Wistar rats by Uyghur medicine. The differences in metabolites were detected through the metabonomics method. Results： The urine of rats in abnormal sapra syndrome group showed significant high abundance metabolites as follows： Leucine, isoleucine, and glycoprotein. And that significant low abundance metabolites as follows： Glutamine, creatine, citric acid, and phenylalanine. Conclusion： The urine of rats with the abnormal sapra syndrome displays abnormal energy metabolism. It is likely that the dysfunctional metabolisms of three major nutrients might be the molecular basis for the abnormal sapra syndrome.