KLF4过表达抑制人白血病K562细胞系的增殖及迁移

Over-expression of KLF4 inhibits the proliferation and migration of K562 cell line

目的探索人锌指转录因子Krüppel样因子4(KLF4)过表达对人慢性髓系白血病细胞K562增殖及迁移能力的影响。方法设稳定过表达KLF4的白血病K562细胞系作为实验组(命名为K562-KLF4细胞),空白K562细胞及空质粒转染的K562细胞(命名为K562-C1细胞)作为对照组。实时荧光定量PCR检测各组细胞KLF4mRNA的表达含量;Western blot检测各组细胞KLF4的蛋白相对表达含量;MTS法检测各组细胞的增殖水平;Transwell检测各组细胞的迁移能力。结果 K562-KLF4细胞KLF4 mRNA的相对表达量比2个对照组明显增高(P<0.05);与对照组相比,K562-KLF4细胞KLF4蛋白相对表达含量明显增加(P<0.05),其过表达率为77.78%;K562-KLF4细胞增殖和迁移能力明显被抑制(P<0.05)。结论 KLF4过表达能抑制K562细胞的增殖及迁移能力。

Objective To study the effect of over-expression of KLF4 on the proliferation and migration ability of K562 cells.Methods The K562 cells with KLF4 over expression（K562-KLF4 cells） were used as experimental group,and paralleled with the vector control（K562-C1 cells） and blank K562 cell control.Real-time PCR was performed to detect the relative expression quantity of KLF4 mRNA of each group cells respectively; Western blot was performed to detect the level of KLF4 protein of each group cells respectively.The cell proliferation was tested by MTS assay.The migration ability of K562 cells was detected by Transwell.Results Compared with K562-C1 cells and blank K562 cells,the relative expression of KLF4 mRNA of K562-KLF4 cells was significantly increased（P〈0.05）.The level of KLF4 protein of K562-KLF4 cells was also significantly increased,by 77.78%（P〈0.05）.The proliferation ability and migration ability of the K562 cells with over-expressing KLF4 were inhibited significantly（P〈0.05）.Conclusions Over-expression of KLF4 could inhibit the proliferation and migration ability of K562 cells.