子宫颈鳞状细胞癌中过氧化物酶体增殖物激活受体γ、维甲酸类X受体α及环氧合酶2的表达及其意义

Expressions of peroxisome proliferator-activated receptor γ, retinoic acid X receptor α and cyclooxygenase 2 in cervical squamous cell carcinoma and their significances

目的 探讨过氧化物酶体增殖物激活受体γ（PPARγ）、维甲酸类X受体α（RXRα）和环氧合酶2（COX-2）在子宫颈鳞状细胞癌（SCC）发生、发展中的可能作用.方法 用免疫组织化学EliVision plus两步法检测17例正常子宫颈鳞状上皮（NCE）、72例子宫颈鳞状上皮内病变（SIL）及42例子宫颈浸润性SCC组织中PPARγ、RXRα和COX-2蛋白的表达情况.结果 PPARγ蛋白在NCE、SIL和SCC组的阳性表达率分别为23.5%（4/17）、58.3%（42/72）和83.3%（35/42）,RXRα蛋白的阳性表达率分别为29.4%（5/17）、54.2%（39/72）和90.5%（38/42）,COX-2在NCE中无明显表达,而在SIL和SCC中的阳性表达率分别36.1%（26/72）和57.1%（24/42）.在SIL中,高级别SIL组PPARγ、RXRα和COX-2的阳性表达率均高于低级别SIL组（均P〈0.05）.在SCC中,有淋巴结转移组COX-2阳性表达率高于无淋巴结转移组（χ2=3.98,P=0.04）.结论 PPARγ、RXRα和COX-2可能均参与了SCC的癌变过程.

Objective To study the functions of peroxisome proliferator-activated receptorγ（PPARγ）, retinoic acid X receptor α （RXRα） and cyclooxygenase 2 （COX-2） in the carcinogenesis of cervical squamous cell carcinoma （SCC）. Methods The expressions of PPARγ, RXRα and COX-2 in 17 cases of normal cervical epithelium （NCE）, 72 cases of squamous intraepithelial lesion （SIL） and 42 cases of cervical SCC were detected with immunohistochemical method respectively. Results The positive expression rates of PPARγ in the NCE, SIL and cervical SCC group were 23.5 % （4/17）, 58.3 % （42/72） and 83.3 % （35/42） respectively; the positive expression rates of RXRα in the NCE, SIL and cervical SCC group were 29.4 %（5/17）, 54.2 % （39/72） and 90.5 % （38/42） respectively. No significant expression of COX-2 was found in the NCE, while the positive expression rates of COX-2 in SIL and cervical SCC were 36.1 % （26/72） and 57.1 %（24/42） respectively. The positive expression rates of PPARγ, RXRαand COX-2 in high-grade SIL group were higher than those in low-grade SIL group （all P〈0.05）. In cervical SCC group, the positive expression rate of COX-2 with lymph node metastasis was higher than that without lymph node metastasis （χ2= 3.98, P= 0.04）. Conclusion PPARγ, RXRαand COX-2 might be all involved in the carcinogenesis of SCC.