非结构蛋白4B与卵巢癌免疫反应抗原域蛋白2相互作用结构域的生物信息学分析

Bioinformatics analysis of interaction domain between NS4B protein and OCIAD2 protein

目的分析非结构蛋白4B(NS4B蛋白)与卵巢癌免疫反应抗原域蛋白2(OCIAD2)相互作用的结构域。方法进入(美国国立生物技术信息中心(NCBI)主页分别查询NS4B蛋白和OCIAD2蛋白Gen Bank序列号,进入通用蛋白质数据库(Uni Prot)主页输入在NCBI中查询的NS4B蛋白和OCIAD2蛋白序列号,查询蛋白编号,进入ELM主页面,输入在Uni Prot查询到的NS4B蛋白编号A4GSN1和OCIAD2蛋白编号Q56VL3,查询跨膜结构域和线性基序。返回ELM主页,选择ELM binding domains,查询数据库里总的相互作用结构域。进入SMART主页,输入蛋白序列号或蛋白序列,查询蛋白跨膜区域和低复杂性区域。低复杂性区域的氨基酸会呈现出弹性的结构容易作为该蛋白和其他类型蛋白相结合的结构域。不同的跨膜区域因共同定位于细胞膜上可能发生相互作用。结果NS4B蛋白存在跨膜区域,OCIAD2蛋白不存在跨膜区域,没有发现NS4B和OCIAD2蛋白存在直接相互作用的线性基序。OCIAD2蛋白具有一个低复杂性区域(88~98氨基酸);NS4B蛋白具有2个低复杂性区域(106~132氨基酸和141~154氨基酸)。结论 OCIAD2蛋白与NS4B蛋白可能通过低复杂性区域发生直接相互作用。

Objective To analyze the interaction domains between nonstructural protein 4B（ NS4B） protein and ovarian cancer immuno-reactive antigen domain containing 2（ OCIAD2） protein. Methods Transmembrane regions and linear motifs were identified by searching the homepage of NCBI（ National Biotechnology Information Center） for NS4 B protein and OCIAD2 protein Gen Bank sequence numbers,entering the Uni Port（ universal protein） home page to input the NS4 B protein and OCIAD2 protein sequence numbers obtained in NCBI and to get the OCIAD2 protein number A4GSN1 and OCIAD2 protein number Q56VL3 in Uni Port,and then entering the ELM home page to input the NS4 B protein number A4GSN1 and the OCIAD2 protein number Q56VL3. Total interaction domains were sought by going to the ELM home page and selecting ELM binding domains. The protein transmembrane regions and the low-complexity regions were identified by entering the SMART home page and entering the protein sequence number,or protein sequence. Low-complexity regions exhibited an elastic structure that readily served as a domain for binding other proteins. Different transmembrane regions bond together because of their colocalization in the cell membrane. Results Transmembrane region was present in NS4 B protein,but not in OCIAD2 protein. No linear interaction motif between NS4 B protein and OCIAD2 protein was identified.OCIAD2 protein had a low-complexity region（ 88-98 amino acids）. NS4 B protein had two low complexity regions [（ 106-132 amino acids） and（ 141-154 amino acids） ]. Conclusion OCIAD2 protein and NS4 B protein may interact directly through low-complexity regions.