腐乳毛霉SH12生长性能及产酶特性研究

Study on Growth Performance and Enzyme Production Property of Mucor SH12 from Sufu

研究了腐乳毛霉SH12的生长性能及产酶特性。结果表明:在温度26℃、pH为7.5时,生长速率最大。Ca^(2+),Mg^(2+)浓度低于0.2%时,对毛霉的生长几乎没有影响。在36h时,毛霉菌丝体干重达到较大值。产酸性蛋白酶、中性蛋白酶、碱性蛋白酶、糖化酶、脂肪酶活力分别为67.3,339.4,372.3,293.1U/g,0.67U/mL,以蛋白酶为主。在豆腐培养基上中性蛋白酶和碱性蛋白酶活力在48h达到最大,而酸性蛋白酶活力在60h达到最大。研究结果可为毛霉SH12用于腐乳生产提供理论依据。

The growth performance and enzyme production property of Mucor SH12 from sufu are investigated. The results indicate that the growth rate is the highest when the temperature is 26 ℃ and pH is 7.5. It has almost no effect on the growth of Mucor when Caz＋ and Mgz＋ concentration is lower than 0. 2%. Mucor mycelium dry weight reaches a larger value at 36 h. The activities of acidic proteinase, neutral proteinase, alkaline proteinase, glucoamylase and lipase biosynthesized by Mucor are 67.30,339.40,372.30,293. 10 U/g and 0.67 U/mL respectively, and proteinases are dominant enzymes. Moreover, the activities of neutral proteinase and alkaline proteinase reach the peak values at 48 h when the Mucor grows on tofu medium, while it is 60 h for acidic proteinase. The results obtained may provide theoretical basis for applying Mucor to produce sufu.