罗平小黄姜多糖提取工艺的优化及抗氧化活性测定

Optimization of Extraction Process and Determination of Antioxidant Activity of Polysaccharides from Luoping Yellow Ginger

采用水提醇沉法提取罗平小黄姜中的多糖,正交试验显示最佳提取工艺为料液比1∶20、提取温度90℃、提取时间160min,在此条件下罗平小黄姜多糖的得率达到6.16%。此外,当多糖浓度达到1.0mg/mL时,对DPPH·和·OH的清除率分别可达57.1%和44.5%,清除DPPH·的IC_(50)为0.96mg/mL。

The polysaccharides of Luoping yellow ginger are extracted by water-extraction and alcohol- precipitation method. The optimal extraction conditions are determined by orthogonal test as follows. Solid-liquid ratio is 1 ： 20, extraction temperature is 90 ℃, extraction time is 160 min. Under the optimum conditions, the yield of Luoping yellow ginger reaches 6. 16%. In addition, when the polysaccharides concentration reaches 1.0 m_g/mL, the scavenging rates of DPPH· and · OH are 57. 1%and 44.5% respectively, and the IC50 of scavenging DPPH · is 0.96 mg/mL.