QuEChERS结合气相色谱-串联质谱法测定水果中5种植物生长调节剂

Determination of five plant growth regulators in fruits by QuEChERS with gas chromatography-tandem mass spectrometry

目的通过优化前处理方法和气相色谱-串联质谱(GC-MS/MS)仪器参数,建立QuEChERS结合GC-MS/MS法同时测定4种水果中4-氯苯氧乙酸、2,4-二氯苯氧乙酸、萘乙酸、吲哚乙酸、吲哚丁酸5种植物生长调节剂残留量的分析方法。方法苹果、橘子、香蕉、葡萄样品经二氯甲烷提取,加入三氟化硼甲醇溶液后选用乙二胺-N-丙基硅烷(PSA)+C18进行净化,加温衍生后,采用GC-MS/MS法对5种植物生长调节剂进行定性、定量分析。结果采用改进的QuEChERS前处理方法,5种植物生长调节剂在10.00~1 000μg/L浓度之间线性良好(r≥0.999),在10.0~200μg/kg添加水平下,方法的回收率在63.4%~107%之间,相对标准偏差在0.480%~14.8%之间。结论该方法快速、准确,结合GC-MS/MS法能满足水果中5种植物生长调节剂残留的检测分析要求。

Objective To establish a method for the determination of 4-chlorophenoxyacetic acid,2,4-dichlor-ophenoxyacetic acid,naphthylacetic acid,indole acetic acid and indolybutyric acid in fruits by QuEChERS with gas chromatography-tandem mass spectrometry（ GC-MS/MS）. Methods Five plant growth regulators in fruits were extracted by methylene chloride,purified by QuEChERS,and were identified and quantified by GC-MS/MS after derivatization from the purified samples. Results The linear correlation coefficients of each linear regression equation for the five plant growth regulators were over 0. 999 and the relative standard deviations（ n = 6） were between 0. 480% and 14. 8%. The recoveries of the method were between 63. 4% and 107% at the spiked levels of 10. 0-200 μg/kg. Conclusion The method was rapid and accurate,and could meet the requirement for the detection of the five kinds of plant growth regulators in fruits.