加味威草胶囊对尿酸性肾病大鼠肾组织NF-κBp50、COX-2蛋白及mRNA的影响

Effects of Jiawei Weicao Capsule on expression of mRNA and protein of NF-κBp50,COX-2 in the renal tissue of rats with urinary acid nephropathy

目的研究加味威草胶囊对尿酸性肾病大鼠肾组织核转录因-κBp50(NF-κBp50)、环氧化酶-2(COX-2)蛋白及mRNA的影响。方法将36只35日龄的SPF级SD随机分为空白组、模型组、加味威草胶囊低剂量组、加味威草胶囊中剂量组、加味威草胶囊高剂量组、依托考昔组,每组6只。除空白组外,其余组均以腺嘌呤+酵母粉混合饲料喂养的方式复制尿酸性肾病大鼠模型。造模成功后,空白组每日给予蒸馏水10 m L/kg灌胃;依托考昔组每日给予依托考昔3.3 mg/kg灌胃,加味威草胶囊低、中、高剂量组分别每日给予加味威草胶囊0.3,0.6,1.2 g/kg灌胃,均连续4周。检测各组大鼠血清尿酸(UA)、尿素(Urea)、肌酐(Cr)水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平。结果模型组及各给药组血清UA、Urea、Cr水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平均明显高于空白组(P均<0.05),加味威草胶囊各剂量组血清UA、Urea、Cr水平和肾组织中NF-κBp50、COX-2蛋白及mRNA表达水平均明显低于模型组(P均<0.05),加味威草胶囊中、高剂量组血清UA、Urea、Cr水平及肾组织中NF-κBp50、COX-2蛋白水平均明显低于依托考昔组(P均<0.05),加味威草胶囊中、高剂量组血清UA、Urea、Cr水平及肾组织中NF-κBp50蛋白表达水平均明显低于加味威草胶囊低剂量组(P均<0.05),加味威草胶囊高剂量组COX-2蛋白和NF-κBp50、COX-2mRNA表达水平均明显低于加味威草胶囊低剂量组(P均<0.05)。结论尿酸性肾病大鼠肾脏中存在NF-κBp50、COX-2的激活,加味威草胶囊对二者有一定的抑制作用,推测抑制NF-κBp50、COX-2的激活是加味威草胶囊抑制肾脏炎症反应、保护肾脏的重要机制之一。

Objective It is to study the effect of Jiawei Weicao Capsule on the expression of mRNA and protein of NF-κBp50,COX-2 in the renal tissue of rats with urinary acid nephropathy( UAN). Methods Thirty-six 35 days old SD rats were randomized to blank group,model group,Jiawei Weicao Capsule of high,middle and low dose groups,Etocoxib group,each group had 6 rats. All the rats except in blank group were fed with adenine + yeast mixed feed to establish UAN models. After model successfully established,the blank group was given distilled water 10 m L/kg by lavage per day,Etocoxib group was given Etocoxib 3. 3 mg/kg by lavage per day,Jiawei Weicao Capsule of low,middle and high dose groups were given 0. 3,0. 6,1. 2 Jiawei Weicao Capsule respectively by lavage,all the groups were treated for 4 weeks. The levels of serum uric acid( UA),urea( Urea),creatinine( Cr) and expression of mRNA and protein of NF-κBp50,COX-2 in the renal tissue of rats in every group were detected. Results The levels of serum UA,Urea,Cr,the expression of mRNA and protein of NF-κBp50,COX-2 in the renal tissue were significantly higher in the model group and treatment groups than those in the blank group( P <0. 05). Compared with the model group,the levels of serum UA,Urea,Cr,the expression of mRNA and protein of NF-κBp50,COX-2 in the renal tissue were significantly decreased in every Jiawei Weicao Capsule group( P < 0. 05). Compared with the etoricoxib group,the levels of UA,Urea,Cr,the expression of protein of NF-κBp50,COX-2 were decreased in Jiawei Weicao Capsule of middle and high dose groups( P < 0. 05). Compared with low dose of JIawei Weicao Capsule group,the levels of UA,Urea,Cr and the expression of NF-κBp50 were decreased significantly in Jiawei Weicao Capsule of middle and high dose groups( P < 0. 05),the expression of COX-2 and mRNA of NF-κBp50 and COX-2 were decreased in high dose of Jiawei Weicao capsule group( P < 0. 05). Conclusion NF-κBp50 and COX-2 were activated in the kidney of urinary acid nephropathy rats. It is presumed that inhibition of NF-κBp50 and COX-2 activation and inflammation is an important mechanism of Jiawei Weicao Capsule for protecting the kidney.