1,8-cineol作为流感疫苗佐剂提高流感疫苗免疫效果的实验研究

1,8-cineol based influenza vaccine can improve vaccine efficacy on mice infected influenza virus

目的:评价1,8-cineol作为流感疫苗佐剂提高流感疫苗免疫效果的作用。方法:将80只BALB/c小鼠分为空白对照组、1,8-cineol组、HA组以及HA+1,8-cineol组,每组20只。空白对照组正常饲养,HA组接种0.2μg HA、HA+1,8-cineol组接种(0.2μg HA+100 mg/kg 1,8-cineol)、1,8-cineol组接种(100 mg/kg 1,8-cineol)。除空白组外,每组小鼠分别在第0、7、14天滴鼻免疫。第21天乙醚轻度麻醉小鼠,以10LD50流感病毒FM1感染每组10只小鼠。连续观察6 d后,每组处死10只小鼠,收集血清,采用酶联免疫法(ELISA)检测血清中IgG、IgG1b、IgG2a、IgG2b在450 nm的吸光值(OD值)。摘取肺组织,计算肺指数,检测肺病理。每组剩余10只小鼠感染15LD50FM1,观察15 d,记录感染鼠的死亡情况,计算感染鼠的生存率。结果:与HA组相比,HA+1,8-cineol组能降低感染鼠的肺指数,缓解感染鼠的肺部病变,能够提高血清中IgG、IgG1b、IgG2a的抗体水平,提高感染鼠的生存率。结论:1,8-cineol作为疫苗佐剂,能够加强流感疫苗的免疫效果。

Objective：To evaluate 1,8-cineol as vaccine adjuvants to enhance influenza vaccine immune efficacy.Methods：80 BALB/c mice were divided into blank control group,1,8-cineol group,HA group and HA＋ 1,8-cineol group,each group of 20.Blank control group normal breeding,HA group accepted HA protein 0.2 μg,the HA＋1,8-cineol group was given 0.2 μg HA＋ 100 mg/kg1,8-cineol,1,8-cineol group was given 100 mg/kg 1,8-cineol.Except the blank group,other groups of mice intranasal immunized for three times interval of one week.At the fourth weeks each mice was infected with 10 LD50 influenza virus FM1.On the 6 days after the infected,10 mice in each group were collected serum,using enzyme-linked immunoassay（ELISA） to detect serum IgG,IgG1 b,IgG2 a,IgG2 b in 450 nm absorbance values（OD）.Removed the lung tissue and measured the lung index and pathological.Remaining mice continue to observe 15 days and record the deaths,calculating the infected mice survival rate.Results：Compared with the HA group,HA ＋1,8-cineol group can reduce the lung index,alleviate the pulmonary lesions,improve the serum IgG,IgG1 b,IgG2 a,and increases the survival rate of infected mice.Conclusion：1,8-cineol as vaccine adjuvants,can strengthen the immune effect of influenza vaccine.