非酒精性脂肪性肝病患者和正常人群的唾液菌群的研究

Analysis in saliva microbiome composition in patients with NAFLD and normal subjects

目的分析非酒精性脂肪肝患者(NAFLD组)与正常对照组(HC组)唾液菌群的多样性。方法收集24例脂肪肝患者和22例正常志愿者的唾液标本,提取总DNA,用PCR扩增16S rDNAV4-V5区,并进行高通量测序,将测序数据进行OTU聚类、多样性分析及物种注释等。结果两组纳入对象的唾液菌群获得的序列平均有39 118条,其中NAFLD组聚类OTUs约308±48,HC组聚类OTUs约305±53。多样性分析NAFLD组和HC组示整体唾液菌群的多样性方面差异较小。物种分析数据表明唾液菌群在门及属水平的组成基本相同,但相对丰度不同,NAFLD组在门水平示厚壁菌门(20.42%)丰度比例升高,拟杆菌门(36.75%)比例降低;属水平的梭菌属、卟啉单胞菌属、韦永氏球菌属的比例较高,而普氏菌属、普雷沃菌属比例显著低。结论 NAFLD组相较于HC组的唾液中厚壁菌门的菌群丰度相对升高而拟杆菌门的菌群丰度相对减少,推测唾液菌群失衡与NAFLD发病可能存在一定关系。

Objective To analyze the diversity of salivary bacteria in patients with nonalcoholic fatty liv-er disease(NAFLD group)and healthy control group(HC group). Methods Saliva samples were collected from24 patients with nonalcoholic fatty liver disease and 22 healthy control. Genomic DNA of the samples was extractedand extended to 16 S rRNA V4-V5 hypervariable region after PCR with Illumina high-throughput sequencing tech-nology. All the data,including operational taxonomic units,diversity indexes and species annotation,etc. was ana-lyzed. Results 39118 optimized sequences in the saliva of each group were obtained,including nonalcoholic fattyliver group clustering OTUs about 308 ± 48 and healthy control group clustering OTUs about 305 ± 53. The diversi-ty analysis of NAFLD group and HC group showed that there was little difference in diversity of the whole salivarybacteria. The data of the species analysis of the saliva bacteria in two group indicate that the composition was simi-lar and the relative abundance was different in phylum and genus. In NAFLD group,the ratio of the abundance offirmicutes(20.42%)increased and the proportion of bacteroides(36.75%)decreased in phylum. Genus of theNAFLD group,the ratio of fusobacterium,porphyromonas and veillonella increased significantly,and the ratio ofprevotella and[Prevotella]decreased. Conclusion In the NAFLD group,the abundance of firmicutes increased,and the abundance of the bacteria decreased. It was speculated that the imbalance of the salivary bacteria is associ-ated with nonalcoholic fatty liver disease.