巨噬细胞集落刺激因子在宫颈癌及宫颈上皮内瘤变患者血清中的表达及意义

The expression and significance of macrophagecolony stimulating factor in patients with cervical lntraepitheliai neoplasia or cervical cancer

目的检测巨噬细胞集落刺激因子(M-CSF)在宫颈癌及宫颈上皮内瘤变(CIN)患者血清中的表达水平,探讨其在宫颈癌发展过程中的作用及早期诊断中的价值。方法选择2014年6月至2016年6月间确诊的宫颈癌患者49例、CIN患者60例、健康体检者40例为研究对象,采取血清标本,分别运用ELISA及MEIA法检测M-CSF、SCC-Ag表达水平。结果 M-CSF和SCCAg在宫颈癌患者血清中表达水平显著高于健康对照组及CIN组(均P<0.01)。M-CSF在CIN组的表达显著高于正常对照组(P<0.05),且高级别CIN组显著高于低级别CIN组(P<0.05),但SCC-Ag在CIN组及正常对照组的表达差异无统计学意义(P>0.05)。联合检测SCC-A和M-CSF显著提高宫颈癌检出率。M-CSF在宫颈癌及高级别CIN患者术后均降低至正常水平,手术前后相比差异有统计学意义(P<0.05),SCCAg则只有在宫颈癌组手术前、后表达差异有统计学意义(P<0.05)。结论 M-CSF可能成为宫颈癌和CIN诊断中有用的血清学指标,可望用于预测CIN和宫颈癌的转归。

Objective To determine the levels of plasma M-CSF in cervical cancer patients and cervicalintraepitheliai neoplasia(CIN)patients,and to discuss the diagnostic utility of M-CSF in the development of cervi-cal cancer. Methods Forty-nine cases of cervical cancer,60 cases of CIN and 40 healthy controls were selectedfrom June 2014 to June 2016. Plasma levels of M-CSF and SCC-Ag were determined by using ELISA and MEIA,respectively. Results Significantly different concentrations of M-CSF and SCC-Ag were observed in patients withcervical cancer compared to the healthy controls and CIN patients(P < 0.01,respectively). Significant differencein plasma M-CSF level between the healthy controls and benign lesion patients were also found(P < 0.05). However,no significant difference in SCC-Ag was found between the healthy controls and benign lesion patients. SCC-Agcombined M-CSF could obviously increase the detection rate of cervical cancer,without increasing the false posi-tive rate. The level of M-CSF in patients with cervical cancer and high grade of CIN reduced to the normal level,with significant difference before and after surgery(P < 0.05). Meanwhile,the level of SCCAg in CIN patients wassignificantly reduced before and after surgery(P < 0.05). Conclusions This study suggests the potential utility ofM-CSF as a good candidate for a serum marker of cervical cancer,as well as benign lesions of this organ(CIN),and M-CSF may also be use to predict the outcome of CIN.