西藏青稞品种甘农大7号白粉菌诱导早期应答基因SSH文库的构建及分析

Construction and Analysis of SSH Library of the Tibetan Hull-less Barley (Hordeum vulagare L. var. nudum HK. f.) Variety Gannong Da 7 in Response to Erysiphe graminis

以接种白粉菌后24 h甘农大7号材料的叶片为实验组(tester),未接种白粉菌材料的叶片为驱动组(driver),分别提取c DNA,利用抑制差减杂交技术,构建"甘农大7号"白粉菌诱导早期应答基因差减文库。对所获得的EST序列去污染后,通过与核酸和蛋白数据库进行序列同源性比对,发现其中有219条非重复序列与已知序列同源性较高。对这些序列进行GO和KEGG功能分析,发现这些注释的基因主要在信号转导、基础物质代谢等方面发挥作用。本结果为后续探究青稞品种甘农大7号抗白粉病的分子机制提供了方向及理论依据。

We used the leaves of the Tibetan hull-less barley（Hordeum vulagare L. var. nudum HK. f.） variety Gannong Da 7 inoculated with powdery mildew for 24 h as the experimental group（tester）, while the non-inoculated leaves served as the driver（control）. Then we extracted c DNAs from the two material groups to construct Gannong Da 7 SSH library by using suppression subtractive hybridization technology. The obtained c DNA sequences in this library were compared with those in Gen Bank. We randomly selected 274 positive clones for sequencing, resulting in 233 high quality ESTs. The analyses of Blast2 GO and KEGG revealed that these genes function in constituting cellular components, photosynthesis, and signal transduction. This work sheds new lights on the direction of our future research, and builds a theoretical basis on further studying the molecular mechanisms of the resistance to powdery mildew in the highland barley variety Gannong Da 7.