hTERC基因表达与宫颈上皮内瘤变进展关系的临床研究

Clinical study on the relationship between hTERC gene expression and the progression of cervical intraepithelial neoplasia

目的研究hTERC基因在不同级别的宫颈上皮内瘤变组织中的表达,探讨hTERC基因与宫颈上皮内瘤变自然进展的关系。方法将我院收治的118例CIN I和CIN II级患者纳入研究,根据随访结果将其分为进展组(21例)、持续组(45例)及逆转组(41例),采用FISH技术检测各组hTERC基因表达情况。结果 CIN I患者hTERC基因异常扩增阳性率为55.93%(33/59),CIN II患者为77.08%(37/48),组间比较,差异显著(P<0.05)。病变进展组总hTERC基因阳性率为95.24%(20/21),持续组为73.33%(33/45),逆转组为36.59%(15/41),组间比较,差异显著(P<0.05)。结论 hTERC基因的阳性表达可作为宫颈上皮内瘤变进展的预测指标,指导临床合理治疗CIN I/II病变。

Objective To study the expression of hTERC gene in different levels of cervical intraepithelial neoplasia,and to explore the relationship between hTERC gene and natural progress of cervical intraepithelial neoplasia. Methods One hundred and eighteen cases of CIN I and CIN II patients treated in our hospital were selected, which were divided into the progress group（21 cases）, the continuous group（45 cases） and the regress group（41 cases） according to the results of follow-up. FISH was used to detect the expression of hTERC gene in each group before the follow-up. Results CIN I patients＇ hTERC abnormal amplification rate was 55.93%（33/59）, CIN II patients＇ was 77.08%（37/48）, there was statistically significant between the two groups（P〈0.05）. The positive rate of hTERC gene was 95.24%（20/21） in the progress group, 73.33%（33/45） in the continuous group and 36.59%（15/41） in the regress group, the differences were significant among the three groups（P〈0.05）. Conclusion The positive expression of hTERC gene can be used as a predictor of progression of cervical intraepithelial neoplasia, which can guide the clinical treatment of CIN I/II lesions.