MicroRNA-195在宫颈癌及子宫上皮瘤变患者外周血中的表达及临床意义

Expression of circulating microRNA-195 and its clinical significance in patients with cervical cancer and cervical intraepithelial neoplasia

目的:研究宫颈癌和子宫上皮瘤变(cervical intraepithelial neoplasia,CIN)患者外周血中微小RNA-195(microRNA-195,miR-195)的表达水平及其临床意义。方法:收集30例健康对照者、51例CIN患者和51例宫颈癌患者,应用实时定量PCR方法检测其外周血中miR-195表达水平,应用受试者工作特征(ROC)曲线分析miR-195表达对诊断CIN、宫颈癌的意义。通过Kaplan-Meier生存分析评价miR-195对宫颈癌患者预后的影响。结果:宫颈癌患者、CIN患者和健康对照者3组间外周血miR-195表达水平差异有统计学意义(P<0.01),其中宫颈癌患者明显低于其他两组(P<0.01或P<0.05)。ROC曲线分析表明外周血miR-195能够很好地区分宫颈癌患者、CIN患者与健康对照者;miR-195低表达患者总体生存时间明显短于miR-195高表达患者(P=0.016)。结论:宫颈癌和CIN患者外周血中miR-195表达降低,可作为辅助诊断宫颈癌和CIN的分子标志。

Objective To evaluate the expression of circulating microRNA-195 （ miR-195） and itsclinical significance in patients with cervical cancer（CC） and cervical intraepithelial neoplasia （ CIN）.Methods： Real-time quantitative PCR was performed to examine serum miR-195 expression levels in 30 healthy controls, 51 CIN patients and 51 CC patients. Receiver operating characteristic curve （ ROC ）were conducted to assess the value of the level of miR-195 expression in dtients from healthy controls. The impact of miR-195 expression on prognosis in CC patients was assessedby Kaplan-Meier method. Results： There were significant differences in the levels oCC patients, CIN patients and the healthy controls（ P 〈0. 01） ; and the level of CC patientcantly lower than those of the other two groups （P 〈0.01 orP〈0.05）. ROC analyses revealevel of miR-195 expression was a potential biomarker in discriminating CC patients or CINJ patients from controls. Moreover, Kaplan-]Meier analysis demonstrated that miR-195 low-expressed patients had shorter overall survival time than miR-195 high-expressed patients （P =0.016）. Conclusion： The lum miR-195 expression in peripheral blood of patients with cervical cancer and CIN couldmolecular marker for the diagnosis of cervical cancer and CIN.