碱性成纤维细胞生长因子对大鼠Ⅰ度烧伤伤口的促愈合作用

The healing function of basic fibroblast growth factor on the wound of second degree burns in rats

目的:通过碱性成纤维细胞生长因子(bFGF)在大鼠Ⅱ度烧伤伤口中的局部应用,观察其对烧伤伤口愈合和烧伤创面愈合各个阶段中相关因子的作用。方法:10%水合氯醛(3.5mL/kg)腹腔注射麻醉大鼠。采用同体对照的方法,用砝码在其背部以脊柱为对称轴,做2个直径为2cm的烧伤创面,2个创面间距>1.5cm。右边伤口给予不同浓度的bFGF,左边伤口给予等体积的0.9%氯化钠溶液为对照。随机分成3组,每组15只,分别给予125、500和2000ngbFGF。隔天给药1次至第14天。在造模后3d、7d、14d、21d和28d处死大鼠,并取下伤口组织进行包埋切片。采用Masson染色法检测组织中胶原的产生,并用免疫组织化学法检测组织中巨噬细胞标记蛋白(CD68)、基质金属蛋白酶2(MMP-2)、增殖细胞核抗原(PCNA)和转化生长因子β_1(TGF-β_1)的表达。结果:伤口愈合率统计显示bFGF可以促进大鼠皮肤烧伤伤口愈合。Masson染色显示bFGF可以促进伤口肉芽组织形成和胶原产生。免疫组织化学结果显示bFGF可以调节伤口中CD68、MMP-2、PCNA和TGF-β_1的表达。结论:bFGF可以调节伤口中CD68、MMP-2、PCNA和TGF-β_1的表达从而促使大鼠I度烧伤伤口愈合。

Objective： To examine the effect of topical basic fibroblast growth factor（bFGF） on wound healing in a rat skin partial thickness burn wound model. Methods： Female Sprague-Dawley rats were anaesthetized with an intraperitoneal injection of 10% chloralic hydrate（3.5 mL/kg）. Hairs on the rat dorsum were clipped and then two partial thickness burn skin flaps were cut per rat. The two wounds were separated by at least 1.5 cm of unwounded skin. The right-side wounds were treated with different doses of bFGF, while the left-side wounds received equal volumes of saline as controls. Wounded rats were randomly divided into three groups（fifteen rats per group） and they were treated with 125, 500 and 2 000 ng of bFGF in right wounds respectively. bFGF treatments were repeated under anesthesia every other day for 14 days. The tissues were harvested and detected with Masson stain and immunohistochemistry to observe the expression of CD68, MMP-2, PCNA and TGF-β_1. Results： By quantitative assessment of wound healing, we found that bFGF treatment significantly accelerated the rate of burn wound healing. Masson staining show that bFGF promoted the formation of granulation tissue and collagen production of wound. Immunohistochemical staining showed that the levels of CD68, MMP-2, PCNA and TGF-β_1 were significantly regulat in bFGF-treated groups compared to control. Conclusion： These findings suggest that bFGF significantly accelerates the healing of partial thickness burn wound healing by regulating the expression of CD68, MMP-2, PCNA and TGF-β_1 in rats.