摘要
对红肉与白肉火龙果的果实进行转录组测序,通过数据的分析与筛选,得到30个差异表达的MYB类转录因子。运用生物信息学进行了MYB转录因子序列的比对、基因注释分析、进化树分析、基因表达情况分析等,通过qRT-PCR验证,探究红肉与白肉火龙果果实颜色的代谢差异与MYB转录因子之间的调控关系。结果表明:火龙果果肉颜色差异可能受MYB转录因子家族部分成员的调控。
Transcriptome sequencing of red-flesh and white-flesh pitaya fruit was conducted,and the data of the sequences were analyzed,from which 30 MYB transcription factors differentially expressed were selected. The MYB transcription factors were analyzed by using bioinformatics such as alignment,gene annotation analysis,phylogenetic analysis,gene expression analysis,etc. The bioinformatic analysis was verified by qRT-PCR to explore the regulatory relationships between metabolism difference and MYB transcription factors of white-or redflesh pitaya fruit. The results showed that the differences in pitaya flesh color may be regulated by some members of the MYB family of transcription factors.
作者
曾灿彬
徐敏
齐钊
闫臻
熊睿
程玉
阮云泽
汤华
ZENG Canbin;XU Min;QI Zhao;YAN Zhen;XIONG Rui;CHENG Yu;RUAN Yunze;TANG Hua(College of Tropical Agriculture and Forestry/Hainan Key Laboratoty of Sustainable Utilization of Tropical Biological Resources, Hainan University, Hailcou, Hainan 570228 , Chin)
出处
《热带生物学报》
2017年第2期153-158,共6页
Journal of Tropical Biology
基金
国家自然科学基金资助项目(31360364)
海南省重大科技项目(ZDZX2013023)