昆虫病原线虫对南方根结线虫的抑制效果

Suppression Effect of Heterorhabditis bacteriophora on Meloidogyne incognita

为明确昆虫病原线虫、异小杆线虫(Heterorhabditis bacteriophora)Hb SD品系对南方根结线虫的生防潜力,在盆栽条件下,通过测定感染南方根结线虫的番茄苗施用昆虫病原线虫悬浮液后的根结指数、卵囊数和繁殖率等指标的变化来研究该Hb SD对南方根结线虫的抑制效果。结果表明:Hb SD 3个不同的接种时间和浓度对番茄根结指数、卵囊数和繁殖率的影响呈现出显著性差异(P﹤0.05),其中,Hb SD悬浮液施用量6 000头每盆并与南方根结线虫同时接种番茄苗的处理组根上的根结指数、卵囊数和繁殖率(2.67,53.8和19.77),与对照(3.5,82.2和26.81)相比均有很大程度的减少,对根结指数的防效可达31.40%。

In order to understand the biocontrol potential of entomopathogenic nematode Heterorhabditis bacteriophora SD（ Hb SD） on Meloidogyne incognita,we inoculated Hb SD infected juveniles（ IJs） suspension and the2 nd stage juveniles（ J2） of M. incognita onto potted tomatoes to test the changes of oocyst numbers on tomato roots,root knot index（ RKI） and reproduction factor（ Rf） to study the suppression effect of Hb SD on M. incognita. The results showed that Hb SD inoculation at the 3 different inoculation time and 2 concentrations had a significantly different（ P ﹤ 0. 05） effect on the oocyst numbers,RKI and Rf. The RKI,the oocyst number and the Rf of the treatment Hb SD 6 000 IJs ＋ M. incognita were 2. 67,53. 8 and 19. 77 in the tomato roots,which were obviously lower than those of the control（ 3. 5,82. 2 and 26. 81） and inhibited upto 31. 40% of M. incognita in the tomato roots.