摘要
利用紫外线照射对七彩鲑(Salvelinus fontinalis)精子进行灭活处理,使其遗传物质失活,作为同源精子诱导源,与七彩鲑卵子进行受精,采用冷休克方法诱导雌核发育。结果表明:同源精子可以诱导七彩鲑的雌核发育,紫外照射强度为72 m J/cm2;距离为8 cm;冷休克温度为20℃。七彩鲑减数分裂雌核发育倍性测定以鸡(Gallus sp.)红细胞DNA含量(2.5 pg/N)为标准、七彩鲑普通育苗子一代为参照,采用流式细胞术和微卫星标记技术对30尾七彩鲑减数分裂雌核发育鱼苗进行分析,证明了雌核发育鱼苗为雌核发育二倍体。
The use of ultraviolet irradiation on Salvelinus fontinalis sperm quenching treatment, the genetic inactivation,as homologous sperm inducement and witli S. fontinalis eg /s were fertilized by cold shock induced /yno/enesis. The results showed that tlie homologous sperm could induce the S. fontinalis pyno/enes is, the intensity om//cm2; distance of 8 cm ＆ cold shock temperature is -20 i. S. fontinalis meiotic dploidy determination in Gallus sp. . red blood cell DNA content 2. 5 pg/PN standards, S. fontinalisgeneration as a reference, using flow cytometry and micro satellite mariners for thie determinatidivision gynogenetic fry were analyzed. Proof of thie gynogenetic diploid gynogenetic fry for.
出处
《淡水渔业》
CSCD
北大核心
2017年第6期21-25,共5页
Freshwater Fisheries
基金
国家自然科学基金(30972191)
吉林省留学人员创新创业项目(201523)
长春市农业先进实用技术的示范推广项目(20130215)
