甘肃地区汉族和回族脑出血患者α-内收蛋白基因rs4963多态性分析

The analysis of ADD1 gene rs4963 polymorphisms between Hanzu and Huizu populations with cerebral hemorrhage in Gansu

目的分析甘肃地区汉族和回族脑出血患者α-内收蛋白基因rs4963多态性的分布情况。方法对甘肃地区汉族和回族脑出血患者各176例应用血液基因组DNA提取试剂盒方法检测α-内收蛋白基因rs4963多态性,比较rs4963多态性的分布差异。结果汉族组和回族组α-内收蛋白基因rs4963基因型分布、等位基因频率均无明显差异(P>0.05);按性别分层后汉族组与回族组基因型分布和等位基因频率仍均无明显差异(P>0.05)。结论α-内收蛋白基因rs4963多态性在甘肃地区汉族和回族脑出血人群中无民族差异。

Objective To investigate the genetic polymorphisms in the ADD1 gene rs4963（ C/G） polymorphisms in patients with cerebral hemorrhage（ CH） from Han nationality and Hui minority populations in Gansu province,China. Methods A total of 352 subjects（ 176 Hanzu and 176 Huizu patients） were collected from Gansu residents. The DNA was extracted by blood genomic DNA extraction kit,and the tag SNP rs4963 polymorphisms of ADD1 gene was detected by Tm-shift genotyping method for all subjects. Results The results showed the genotype and allele frequency of rs4963 were not significantly different between Hanzu and Huizu CH patients（ P〈0. 05）. The stratification analysis of gender indicated that the genotype and allele frequency of rs4963 were not significantly different between Hanzu and Huizu CH patients（ P〈0. 05）. Conclusion The genetic polymorphisms in ADD1 gene rs4963 were not significantly different between Hanzu and Huizu CH patients in Gansu Province,China.