糖尿病肾病患者抗内皮细胞抗体表达与炎性细胞因子水平关系分析

Correlation analysis of anti-endothelial cell antibody expression and inflammatory cytokines levels in patients with diabetic nephropathy

目的分析糖尿病肾病(DN)患者血液中的抗内皮细胞抗体(AECA)与炎性细胞因子水平的关系。方法选择64例DN患者设为DN组,根据AECA检测结果将其分为AECA阳性组和阴性组,选择34例糖尿病(DM)初诊患者设为糖尿病初诊组,另选择11例健康体检者为健康组,测定所有研究对象的IL-6、TNF-α和P-选择素。结果 DN组炎性细胞因子水平明显高于DM初诊组和健康组(P<0.05);DM初诊组炎性细胞因子水平高于健康组(P<0.05);AECA阳性组炎性细胞因子水平明显高于AECA阴性组,差异显著(P<0.05)。结论炎症造成血管损伤并诱发机体产生自身免疫,AECA介导的免疫损伤又可加重内皮细胞功能障碍,二者相互作用对DN患者产生重要作用。

Objective To analyze lhe correlalion of anli-endolhelial cell anlibody （AECA） expression and inflammalory cylokines levels in palienls wilh diabelic nephropalhy （DN）. Methods Sixly-four DN palienls were selecled as lhe DN group, and all lhe palienls were divided inlo AECA posilive group and AECA negalive group, while 34 diabelic （DM） newly diagnosed palienls were selecled as lhe DM newly diagnosed group, anolher 11 heallhy examined people were selecled as lhe heallhy examined group. The IL-6, TNF-琢 and P-seleclin （PS） were delecled lhrough all lhe sludy objecls. Results The inflammalory cylokines levels of lhe DN group were higher lhan lhose of lhe DM newly diagnosed group and heallhy examined group （P〈0.05）; and lhose of lhe DM newly diagnosed group were higher lhan lhe heallhy examined group （P〈0.05）. The inflammalory cylokines levels of lhe AECA posilive group were higher lhan lhose of lhe ECA negalive group （P〈0.05）. Conclusion Inflammalion causes vascular damage and induces lhe body lo produce auloimmunily, AECA medialed immune damage also aggravaled endolhelial cell dysfunclion, bolh inleraclions play an imporlanl role in DN palienls.