超促排卵后不同取卵时间对昆明小鼠卵子质量、体内成熟率和体外受精率的影响

Different Oocyte Collecting Time Impact on Quality of Oocyte,in Vivo Maturation Rate and in Vitro Fertilization Rate after Superovulation in KM Mice

目的提高超促排卵KM小鼠卵子质量、体内成熟率和体外受精率。方法对100只KM小鼠进行超促排卵,并观察超促排卵后获得卵子的卵核与第一极体位置及卵周隙变化、卵子形态、卵子体内成熟率和体外受精率。结果注射人绒毛膜促性腺激素(hCG)后12 h、14 h取卵,卵子质量最好;注射hCG后13 h所取卵的体内成熟率和体外受精率最高。结论 KM系小鼠体外受精的最佳取卵时间为超促排卵后13 h。

Objective To improve the quality of oocyte, in vivo maturation rate and in vitro fertilization rate in KM mice. Methods One-hundred KM mice were super-ovulated, the changes of the oocyte nucleus and the first polar body position, the perivitelline space changes, oocyte morphology, in vivo maturation rates and in vitro fertilization rates were observed and calculated. Results The oocyte quality was best after 12 h, 14 h of injection with human chorionic gonadotropins （hCG）, and oocyte in vivo maturation rate and in vitro fertilization rate were the highest after 13 h. Conclusion Collection of oocyte after 13 h of superovulation was the best time for in vitro fertilization of KM mouse.