蝙蝠蛾拟青霉和中国弯颈霉蛋白质氨基酸分析及质量评价

Content analysis and nutritional evaluation of amino acids in Paecilomyces hepiali and Tolypocladium sinensis

目的对蝙蝠蛾拟青霉(Paecilomyces hepiali)和中国弯颈霉(Tolypocladium sinenis)2种虫生真菌菌丝的蛋白质氨基酸进行含量分析及质量评价,为2种真菌的其他相关研究和应用提供依据。方法采用盐酸水解法水解菌丝粉,用Agilent 1200高效液相色谱仪C18反相柱梯度进行分离,上柱前加入邻苯二甲醛-氯甲酸芴甲酯(OPA-FMOC)自动衍生化。参照标准品氨基酸,计算待测样品的氨基酸含量。结果测定了17种蛋白水解氨基酸的含量,其中蝙蝠蛾拟青霉中有4种氨基酸(天冬氨酸、缬氨酸、异亮氨酸、亮氨酸)的含量高于中国弯颈霉,中国弯颈霉中有11种氨基酸(谷氨酸、丝氨酸、甘氨酸、苏氨酸、精氨酸、丙氨酸、酪氨酸、胱氨酸、蛋氨酸、赖氨酸、脯氨酸)的含量高于蝙蝠蛾拟青霉。中国弯颈霉氨基酸总量、必需氨基酸总量、非必需氨基酸总量高于蝙蝠蛾拟青霉,蝙蝠蛾拟青霉支链氨基酸含量高于中国弯颈霉。两者必需氨基酸的模式均符合世界卫生组织和联合国粮农组织(WHO/FAO)提出的必需氨基酸模式谱和参考标准。结论蝙蝠蛾拟青霉与中国弯颈霉均具有较高的营养价值,但两者的蛋白质氨基酸含量并不等同。

Objective To analyze the contents of amino acids in Paecilomyceshepiali(P.hepiali)and Tolypocladium sinenis(T.sinensis)and their nutritive values,so as to provide a basis for related researches and applications of the two kinds of fungi.Methods The mycelia of P.hepiali and T.sinensis were hydrolyzed with6 mol·L-1 HCl,and the amino acids from the mycelia were separated by C18 reversed-phase column with gradient elution and o-phthalaldehyde-fluorenylmethyl chloroformate(OPA-FMOC)pre-column derivation using Agilent 1200 high-performance liquid chromatograph.The content of sample amino acids was determined by the peak areas ratio between the samples and standard amino acids.Results A total of 17 proteolytic amino acids from P.hepiali and T.sinensis were determined in this study.The contents of 4 kinds of amino acids(aspartic acid,valine,isoleucine and leucine)in P.hepiali were higher than those in T.inensis,while the contents of 11 kinds of amino acids(glutamic acid,serine,glycine,threonine,arginine,alanine,tyrosine,cystine,methionine,lysine and proline)in T.inensis were higher than those in P.hepiali.The total contents of amino acids,essential amino acids and non-essential amino acids in T.Sinensis were higher than those in P.hepiali,and the content of branched chain amino acids in P.hepiali was higher that in T.sinensis.World Health Organization/Food and Agriculture Organization(FAO/WHO)reference model of essential amino acids was used to evaluate the nutritional values of amino acids in P.hepiali and T.sinensis,and the results showed that the scores of ratio coefficient of amino acids in P.hepiali and T.sinensis were 92.06 and 88.17,respectively.Conclusion The contents of protein amino acids in P.hepiali and T.sinensis are different,but they both have relatively high nutritional values.