硒矿区土壤中真菌的分离培养及抗肿瘤活性筛选

Isolation of Fungal Strains in Soil of Selenium Mining Area and Screening for Their Anti-tumor Activity

利用单菌落挑选与划线培养技术,从硒矿区土壤样品中分离纯化真菌。通过摇床发酵培养,提取制备发酵样品。采用MTT法,用MDA-MB-231细胞、Hela细胞、SMMC-7721细胞测试发酵样品的抗肿瘤活性。从采自湖北恩施全球唯一独立硒矿床区的6个不同土样中分离真菌73株。其中,对人宫颈癌Hela细胞的IC_(50)小于90μg/m L的菌株32株,对人乳腺癌MDA-MB-231细胞的IC_(50)小于90μg/m L的菌株13株,对人肝癌SMMC-7721细胞的IC_(50)小于90μg/m L的菌株9株。这些活性菌株为筛选抗肿瘤天然产物提供了良好的药源菌株。

The fungal strains were obtained by single colony isolation technique from the soil of the world＇s unique proved independent selenium deposit. The anti-tumor activity was assayed by MTT method using MDAMB-231 cells, Hela cells and SMMC-7721 cells, respectively, together with morphological observation of these cells under inversed light microscope. The 73 terrestrial fungal strains were isolated from the soil samples collected from 6 different areas in Enshi area of Hu Bei Province, China. Among them, 32 strains produced fermentation products obviously inhibiting the Hela cells, 13 strains inhibiting the MDA-MB-231 cells and 9 strains inhibiting the SMMC-7721 cells with IC50 value less than 90 μg/ml. These antitumor-metabolite-producing strains have provided fungal strains for further studies on the bioactive metabolites.