游泳运动对APP/PS1转基因小鼠学习记忆能力的影响

Effects of Swimming Exercise on Learning and Memory Ability of APP/PS1 Transgenic Mice

探讨游泳运动对APP/PS1转基因小鼠学习记忆能力的影响。选择11月龄的雄性APP/PS1转基因小鼠,随机平均分为对照组和游泳组,对照组常规饲养而游泳组进行1个月的游泳运动训练。分别采用刚果红染色、Tunel检测、Western Blot和Morris水迷宫等实验方法观察小鼠大脑皮层Aβ斑形成、神经元凋亡、线粒体生成相关蛋白表达和学习记忆能力的变化情况。结果发现,游泳运动可以减少APP/PS1转基因小鼠大脑皮层Aβ斑的形成、抑制神经元凋亡、促进线粒体生成、增强小鼠的学习记忆能力。由此可见,游泳运动可作为一项防治阿尔茨海默病的行为治疗候选方案。

To investigate the effects of swimming on learning and memory ability of APP/PS1 transgenic mice. 11-month-old male APP/PS1 transgenic mice were randomly divided into control group and swimming group. The control group had no exercise, and the swimming group had swimming training for 1 month. The expression of Aβ plaques, neuron apoptosis, mitochondria biogenesis-related protein expression and the learning and memory of APP/PS1 transgenic mice were determined by Congo red staining, Tunel assay, Western Blot and Morris water maze. The result showed that swimming exercise reduced the formation of Aβ plaques and neuronal apoptosis, promoted mitochondria biogenesis in the cerebral cortex of APP/PS1 transgenic mice, and enhanced the ability of learning and memory of APP/PS1 transgenic mice. This study suggested that swimming can promote the learning and memory ability of APP/PS1 transgenic mice by facilitating mitochondria biogenesis, and thus can be used as a treatment for the prevention and treatment of Alzheimer＇s disease.