小鼠胚胎脑源与脊髓源神经干细胞增殖能力的比较

Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice

目的探讨小鼠前脑源和脊髓源神经干细胞(NSCs)的增殖能力差异.方法选择孕14 d C57BL胎鼠,机械分离法从前脑和脊髓分离培养神经干细胞,Nestin/Sox2免疫荧光染色鉴定NSCs.计算细胞倍增速率(Multiplication rate)和细胞倍增时间(Cell doubling time),Brd U/Nestin双标检测NSCs增殖.结果由C57BL胎鼠前脑和脊髓分离得到的细胞群,均可在体外分裂增殖形成神经球,Nestin&Sox2标记阳性.脑源NSCs增殖速度显著高于脊髓源(P<0.05),而倍增时间则与增殖能力成反比;P5代时两种干细胞增殖最快;Nestin&Brd U双标的NSCs也证明了正在增殖的脑源NSCs(29.65±3.38)%显著多于脊髓源的NSCs(19.93±1.95)%(P<0.05).结论来源于前脑的NSCs增殖能力更强.

Objective To discusses the differences of proliferation between fetal forebrain- and spinalcord-derived neural stem cells（NSCs ） in mice. Methods The NSCs were separated by the mechanical methodfrom the forebrain and spinal cord of 14 day C57BL foetus, and the immunocyfluorescent staining of Nestin ＆ Sox2used to as the NSCs markers. Meanwhile,calculating multiplication rate and cell doubling time, with the additionof BrdU/Nestin double staining were used to test the proliferation. Results Firstly, multiplication rate offorebrain-derived NSCs significantly proliferated faster than spinal cord-derived（ 〈0.05）, and cell doublingtime is inversely proportional to NSCs multiplication. Secondly, the 5 passages of these two sources of NSCs werethe fastest. Lastly,the BrdU＋ NSCs also suggested that the proliferating forebrain-derived NSCs （29.65%±3.38）were significantly more than spinal cord-derived （29.65%±3.38）（ 〈0.05） . Conclusion Our study suggeststhat the forebrain-derived NSCs have more capacity of proliferation.