摘要
以蛋白质清除率和多糖保留率为评价指标,比较Sevage法、TCA法、酶法、Sevage-酶法和TCA-酶法的脱蛋白效率。确定PBPC脱蛋白最适方法为Sevage-酶法,即木瓜蛋白酶浓度2%,酶解时间2 h,pH 7.5,酶解温度50℃,Sevage法4次。该法蛋白清除率45.88%,多糖保留率86.49%。
Taking protein removal rate and polysaccharides retention rate as the evaluation indexes,the Sevage method,TCA method,enzyme method,Sevage-enzyme method and TCA-enzyme method were compared.Among the five methods,Sevage-enzyme method was the optimum deproteinization method.The deproteinization technologic condition was 2% enzyme concentration,pH of 7.5,enzymolysis temperature 50 ℃,and Sevage method repeat 4 times.Under this condition,deproteinized rate and polysaccharides retention rate were 45.88% and 86.49% respectively.
出处
《食品工业》
北大核心
2017年第11期19-22,共4页
The Food Industry
基金
国家自然科学基金资助项目(31460231)
云南省科技厅高新科技计划项目(GX10-2004)
云南省昆虫生物医药研发重点实验室项目(2014-10)
关键词
响应面法
红花蜂花粉多糖
脱蛋白
aking protein
removal rate
polysaccharides retention rate
valuation indexes
Sevage method
TCA method
enzyme method
Sevage-enzyme method
TCA-enzyme method
