摘要
目的通过观察心痛方对大鼠急性心肌梗死(AMI)后基质金属蛋白酶-9(MMP-9)mRNA和金属蛋白酶组织抑制物-1(TIMP-1)mRNA表达及对心肌形态学的影响,探讨心痛方治疗AMI的疗效机制。方法 80只SD大鼠随机分为假手术组、模型组、心痛方组、双抗组(阿司匹林+氯吡格雷),冠脉结扎法制备AMI模型,灌胃给药7 d后处死大鼠,运用RT-PCR法测定心肌组织MMP-9 mRNA、TIMP-1 mRNA表达,心肌细胞HE染色观察形态学改变。结果心痛方能使AMI大鼠心肌内MMP-9 mRNA表达减少(P<0.01);TIMP-1mRNA表达升高(P<0.01);MMP-9 mRNA/TIMP-1 mRNA比值降低(P<0.01);心肌损伤程度及心梗面积与MMP-9 mRNA/TIMP-1 mRNA比值呈正直线相关。结论心痛方对AMI缺血心肌具有保护作用,其作用机制可能与增加TIMP-1 mRNA表达,降低MMP-9 mRNA表达,从而调节MMP-9 mRNA/TIMP-1 mRNA的平衡有关。
Objective： To investigate the effect of Xintong Decoction on the expression of matrix metallopro- teinase-9 （MMP-9） mRNA,tissue inhibitor of metalloproteinase-1 （TIMP-1） mRNA and effects on myocardial morphology in acute myocardial infarction （AMI） rats,and to explore the therapeutic mechanism of Xintong De- coction on AMI. Methods： 80 SD rats were randomly divided into sham operation group （A group）,the model group（group B）,Xintong Decoction group（group C）, and Dual anti-platelet group （aspirin＋clopidogrel, group D）. AMI model was established by coronary artery ligation method. Rats were killed 7 days after intragastric adminis- tration. Expression of MMP-9 mRNA and TIMP-1 mRNA in myocardium were observed by RT-PCR,and mor- phological changes were observed by HE staining. Results： Xintong Decoction could reduce the expression of MMP-9 mRNA（P 〈 0.01 ）,increase the expression of TIMP-1 mRNA（P〈0.01）,and decrease the ratio of MMP-9 mRNA/TIMP-1 mRNA in AMI rats （P〈 0.01 ）. The degree of myocardial injury and the area of myocardial infarc- tion were positively correlated with MMP-9 mRNA/TIMP-1 mRNA ratio. Conclusion： Xintong Decoction has protective effects on AMI ischemic myocardium, and its mechanism may be related to increasing the expression of TIMP-1 mRNA,reducing the expression of MMP-9 mRNA and thus regulating the balance of MMP-9 mRNA/ TIMP-1 mRNA.
出处
《中国中医急症》
2017年第11期1922-1925,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
湖南省教育厅科学研究项目(16A159)
