博落回根中生物碱的分离

Separation of Alkaloids from the Roots of Macleaya Cordata

目的:研究博落回根中的生物碱成分。方法:采用硅胶柱色谱、重结晶、半制备液相色谱制备化合物,利用NMR、MS等波谱技术鉴定化合物结构。结果:从博落回根的乙醇提取物中分离得到9个生物碱类化合物,分别为二氢白屈菜红碱(1),6-氰基二氢血根碱(2),R-6-((R)-1-hydroxyethyl)dihydrochelerythrine(3),碎叶紫堇碱(4),四氢小檗碱(5),8-去甲基二氢白屈菜红碱(6),8-去甲基白区菜红碱(7),原阿片碱(8),别隐品碱(9)。结论:化合物4~6首次在博落回属植物中分离得到。

Objective ： To study the alkaloids in Macleaya cordata. Methods： The compounds were isolated and purified by silica gel, recrystallization, and semi-preparative HPLC, and their strcuctures were confirmed by MS and NMR. Results： Nine alkaloids were obtained and identified as dihydrochelerythrine （ 1 ）, 6-cyanodihydrosanguinarine （ 2 ）, R-6-（ （ R ） -1-hydroxyethyl ） dihydrochel- erythrine （3）, 8-demethyl-dihydrochelerythrine （4）, cheilanthifoline （5）, canadine （6）,8-demethyl - chelerythrine （7）, protopine （8） and allocryptopine（9）. Conclusion： Compounds 4-6 are isolated from the genus of Macleaya for the first time.