雷公藤化学成分研究

Studies on the Chemical Constituents of Tripterygium Wilfordii

目的:研究雷公藤根的化学成分。方法:通过硅胶柱色谱、Sephadex LH-20凝胶色谱和制备高效液相色谱等方法进行化合物分离纯化,根据理化性质和波谱数据确定其结构。结果:从雷公藤根中分离鉴定了12个化合物,分别为:雷公藤吉碱(1)、雷公藤次碱(2)、雷酚萜甲醚(3)、任卡漆-5-烯-3β,28-二醇(4)、雷酚萜E(5)、雷酚萜L(6)、maytenoic acid(7)、雷酚内酯(8)、雷公藤红素(9)、去甲泽拉木醛(10)、1-desacetyl wilforgine(11)、雷公藤春碱(12)。结论:首次对化合物1和2的1D、2D核磁数据进行全归属,并运用X-ray单晶衍射技术确定了化合物1的绝对构型。

Objective： To study the chemical constituents in the roots of Tripterygium wilfordii. Methods： The compounds from Tripterygium wilfordii were isolated and purified by silica gel, Sephadex LH-20 and prep-HPLC chromatography, and their structures were elucidated based on the physiochemical properties and spectroscopic analysis. Results： Twelve compounds were isolated and iden- tified as wilforgine （ 1 ）, wilforine （2）, triptonoterpene methyl ether （3）, glut-5-en-3/3,28-diol （4）, wilforol E （5）, triptobenzene L （6）, maytenoic acid （7）, triptophenolide （8）, celastrol （9）, demethylzeylasteral （10）, 1-desacetyl wilforgine （ 11 ） and wilfortrine （12）. Conclusion： The 1D and 2D NMR data of 1 and 2 are assigned for the first time, and the absolute configurations of 1 are confirmed by X-ray single crystal diffraction.