Determination of Paraquat in Human Serum by HPLC

目的：建立HPLC法测定人血清中百草枯含量。方法：色谱柱：Kromasil C18柱（200mm×4．6mm，5μm），流动相：乙腈-水（含0．03mol·L-1庚烷磺酸钠，0．24mol·L-1磷酸）=3：97（用三乙胺调pH至2．0）；检测波长258nm；柱温25℃；进样体积20μl；流速0．8ml·min-1。结果：百草枯在0．106—10．6mg·L-1浓度范围内线性关系良好（r=0．9993），最低检出浓度为0．065mg·L-1。高、中、低3种浓度样品绝对回收率〉89．4％，方法回收率〉94．4％，日内精密度RSD在0．12％～1．74％之间，日间精密度RSD在0．44％～2．89％之间。结论：该方法操作简便易行、灵敏度高、专属性强，适用于百草枯的人体血清浓度测定。

Objective： To establish an HPLC method for the determination of paraquat in human serum. Methods： The analytical column was a Kromasil C18 column （200mm × 4.6mm,5 μm）. The mobile phase was a mixture of acetonitrile and water （ containing 0.03 mol·L-1 sodium heptanesulfonate and 0.24 mol·L-1 phosphoric acid ） （3 ： 97, pH was adjusted to 2.0 by triethylamine）, the detection wavelength was set at 258 nm, the column temperature was 25℃, the injection volume was 20μl, and the flow rate was 0.8 ml·min-1. Results： The calibration curve of paraquat was linear within the range of 0. 106-10.6 mg·L-1 （ r =0. 999 3） ,and the lower limit of detection was 0.065 mg·L-1. The absolute recovery of paraquat at low, medium and high concentration was more than 89.4% ,and the method recovery was more than 94.4%. The intra-day RSDs were 0. 12%-1.74%, and the inter-day RSDs were 0.44% -2.89%. Conclusion： The method is simple, quick, accurate, sensitive and specific, and can be used for detecting paraquat concentration in human serum.