甲基莲心碱对HepG2细胞增殖及凋亡的影响

Effects of Neferine on Proliferation and Apoptosis of HepG2 Cells

目的:研究甲基莲心碱对人肝癌HepG2细胞增殖及凋亡的影响。方法:采用CCK-8法观察甲基莲心碱对人肝癌细胞HepG2细胞生长增殖的影响;Hoechst33258染色观察甲基莲心碱作用HepG2细胞后细胞形态的变化;测定乳酸脱氢酶(LDH)释放率观察HepG2细胞受损程度;Annexin V-PI双染测定甲基莲心碱对HepG2细胞凋亡率的影响;PI/RNase单染检测不同浓度的甲基莲心碱对其凋亡周期的影响。结果:甲基莲心碱对体外培养的人肝癌HepG2细胞的生长具有抑制作用,且呈剂量、时间依赖性;细胞膜的受损程度随着药物剂量的增大逐步提高;Hoechst33258及流式结果表示,甲基莲心碱可使HepG2细胞阻滞于G_0/G_1期,细胞凋亡率随药物浓度的增大亦呈增长趋势。结论:甲基莲心碱可显著抑制肝癌HepG2细胞的生长增殖,并在一定的范围内呈剂量、时间依赖性,诱导其阻滞于G_0/G_1期,发生晚期凋亡。

Objective： To investigate the effects of neferine on the proliferation and apoptosis of HepG2 cells. Methods： The effect of neferine on the proliferation of HepG2 cells was determined by cell counting kit-8 （ CCK-8）, the morphology of HepG2 cells with Hoechst33258 staining was observed under a fluorescent microscope, the degree of damage to HepG2 cells was observed by lactate dehydrogenase （LDH） kit,Annexin V/propidium iodide （PI） and PI/RNase were used to analyze the apoptosis and the cell cycle. Results： Neferine could inhibit the proliferation of HepG2 cells in a dose and time-dependent manner, and the degree of damage to the cell membrane increased with the dose of the drug. The results of Hoechst33258 staining and flow cytometry （FCM） indicated that HepG2 cells were arrested in G0/G1 phase and the apoptotic rate increased with the concentration increase of neferine. Conclusion： Neferine can inhibit the growth and proliferation of HepG2 cells in a dose and time-dependent manner, induce it arrested in G0/G1 phase and induce the late apoptosis of HepG2 cells.