基于指标成分和药效学优选定痛宁颗粒的醇沉工艺

Optimization of Alcohol Precipitation Technology for Dingtongning Granule Based on Index Components and Pharmacodynamics

目的:优选定痛宁颗粒的醇沉工艺,结合药效学角度考察醇沉工艺的合理性。方法:以干膏得率和芍药苷、阿魏酸转移率的综合评分为指标,通过L_9(3~4)正交试验考察醇沉浓度、清膏相对密度和醇沉时间对醇沉工艺的影响,采用小鼠生化指标变化比较定痛宁颗粒水提清膏醇沉前后的药效。结果:最佳醇沉工艺条件为提取液浓缩至相对密度的清膏1.10 g·ml^(-1)(25℃),加入一定量的90%乙醇使药液含醇量达70%,冷藏静置18 h。醇沉前后药效未发生明显变化。结论:优选的水提醇沉工艺稳定可行,在保持药效的基础上达到了较好的纯化效果,为定痛宁颗粒的科学生产提供了实验依据。

Objective ： To optimize the alcohol precipitation technology of Dingtongning granule and investigate the rationality of alcohol precipitation technology by pharmacodynamics. Methods： With the yield of extract and the transfer rates of paeoniflorin and feru- lic acid as the comprehensive evaluation indices, L9 （3^4） orthogonal test was used to optimize the alcohol precipitation technology by taking the concentration of alcohol precipitation, relative density of extract and alcohol precipitation time as the influencing factors, and the efficacy of water extract before and after the alcohol precipitation was compared through the biochemical indices of mice. Results： The optimum alcohol precipitation process was as follows： the extracting liquid was concentrated to the relative density of 1.10 g·ml-1 （25℃ ）, and then 90% ethanol was added till the concentration of ethanol up to 70%, and last, the extract was stored in cold for 18 h. The efficacy had no obvious changes before and after the alcohol precipitation. Conclusion： The optimal alcohol precipitation technology is stable and feasible, which can maintain the efficacy and shows good purification. The optimal alcohol precipitation technology provides experimental basis for the scientific production of Dingtongning granule.