干扰linc00152抑制胶质瘤细胞增殖与侵袭

Interference with linc00152 can inhibit glioma cell growth and invasion

目的观察干扰linc00152的人胶质瘤细胞株U251的增殖与侵袭情况。方法培养胶质瘤U251细胞,将U251细胞分为观察组和对照组,观察组转染linc00152-sh RNA,对照组转染control-sh RNA,采用q RT-PCR法检测两组U251细胞中linc00152 m RNA的表达,采用MTT法观察两组细胞增殖能力,侵袭实验检测两组细胞的侵袭能力。结果 q RT-PCR结果显示,观察组U251细胞中linc00152的m RNA表达量为(0.313±0.020),明显少于对照组的(1.017±0.082),差异有显著统计学意义(P<0.01);MTT结果显示,观察组的U251细胞的OD值为(0.444±0.032),少于对照组的(0.679±0.052),差异具有统计学意义(P<0.05);侵袭实验结果显示,观察组穿过基质胶的U251细胞数量为(66.9±9.1),明显少于对照组的(146±12.2),差异具有显著统计学意义(P<0.01)。结论在人胶质瘤细胞株U251中敲低linc00152能抑制细胞的增殖与侵袭。

Objective To observe the growth and invasion of human glioma cell line U251 interfered with linc00152. Methods Cultured glioma U251 cells were divided into the observed group and the control group. The ob-served group was transfected with linc00152-shRNA, and the control group was transfected with control-shRNA. The ex-pression of linc00152 mRNA in U251 cells of the two groups was detected by quantitative reverse transcription-PCR （qRT-PCR） method. Tetrazolium-based colorimetric （MTT） assay was used to observe the proliferation ability of cells in the two groups. The invasive ability of the two groups cells was detected by invasion assay. Results QRT-PCR results showed that the expression of linc00152 mRNA of U251 cells in the observed group was （0.313 ± 0.020）, which was signifi-cantly lower than （1.017±0.082） in the control group （P〈0.01）. MTT results showed that the OD value of U251 cells in the ob-served group was （0.444±0.032）, which was significantly less than （0.679±0.052） in the control group （P〈0.05）. Invasion as-say results showed that the number of U251 cells passing through the matrix glue in the observed group was （66.9 ± 9.1）, which was significantly less than （146±12.2） in the control group （P〈0.01）. Conclusion The knockdown of linc00152 in human glioma cell line U251 can inhibit cell proliferation and invasion.