磺胺嘧啶银联合芦荟烧伤膏对糖尿病大鼠末梢循环障碍及足溃疡的保护作用

A study of silver sulfadiazine combined with aloes burn cream in the protection of peripheral curculatory diaorders and ulcer on diabetic foot in diabetic rat

目的研究磺胺嘧啶银联合芦荟烧伤膏对糖尿病大鼠足溃疡的保护作用并探讨其机制。方法选取清洁级性成熟Wistar大鼠90只制成糖尿病大鼠模型,将成功造模的80只大鼠按照随机数表法分为末梢循环障碍模型组与足溃疡模型组,每组均40只。成功造模且符合研究要求的末梢循环障碍模型组(n=35)按照随机数表法分为对照组(n=18)与观察组(n=17),足溃疡模型组(n=36)按照随机数表法分为对照组(n=18)与观察组(n=18),所有大鼠均给予磺胺嘧啶涂抹患处,观察组在此基础上给予芦荟烧伤膏,连续给药35 d,治疗期间(1周、2周、3周)记录末梢循环障碍大鼠足底皮温、疼痛阈值,记录足溃疡大鼠溃疡面愈合情况及其外周血清血管内皮生长因子(VEGF)。结果末梢循环障碍模型组大鼠治疗过程中各个时间点观察组的足底温度高于对照组,而疼痛阈值低于对照组,且差异有统计学意义(P<0.05)。足溃疡模型组大鼠治疗过程中各个时间点比较观察组溃疡愈合率、外周血清血管内皮生长因子(VEGF)均高于对照组,且差异有统计学意义(P<0.05)。结论磺胺嘧啶银联合芦荟烧伤膏能很好地改善糖尿病大鼠的末梢循环引起的温度及痛觉迟钝,与此同时能通过提高VEGF的表达促进足溃疡大鼠溃疡面愈合。

Objective To study the mechanism of silver sulfadiazine combined with aloes burn cream in the protection of peripheral curculatory diaorders and ulcer on diabetic foot in diabetic rats. Methods A total of 80 model-ing diabetic viripotent Wistar rats （modeled from 90 rats） were divided into the mold of peripheral curculatory diaorders group and the ulcer group according to random number tables, with 40 rats in each group. The peripheral curculatory di-aorders group received an ice-cold stimulation, and 35 rats were successful, which were divided into the control group （silver sulfadiazine, n=18） and the observation group （silver sulfadiazine＋aloes burn cream, n=17） randomly and re-ceived different treatments （treatment course for 35 days）. On post treatment day （1, 2, and 3 weeks）, the plantar tempera-ture, hot pain thresholds of rats were detected in the peripheral curculatory diaorders group. Ulcer on diabetic foot group were given suffocating by steam, and 36 rats were successful, which were divided into the control group （silver sulfadia-zine, n=18） and the observation group （silver sulfadiazine＋ aloes burn cream, n=18） randomly and received different treatments （treatment course for 35 days）. On post treatment day （1, 2, and 3 weeks）, the healing condition of ulcer, and the level of vascular endothelial growth factor （VEGF） in the serum were detected. Results During the 35-day treat-ment, for the peripheral curculatory diaorders group, the plantar temperatures in the observation group were significantly higher than that in the control group （P〈0.05）, while the hot pain thresholds in the observation group were significantly lower than that in the control group （P〈0.05）;For the ulcer group, the level of VEGF and the healing condition of ulcer in the observation group were significantly higher than that in the control group （P〈0.05）. Conclusion Silver sulfadia-zine combined with aloes burn cream can improve the plantar temperature and pain dysesthesia of rats caused by diabet-ic peripheral circulatory disorders, and promote the wound healing of diabetic foot ulcers in rats with up-regulation of VEGF, which has the certain guiding value for clinical work.