不同病期慢性阻塞性肺疾病趋化因子受体3和淋巴细胞亚群的表达及其影响

Expression and effect of CXCR3 and lymphocyte subsets at different stages in patients with chronic obstructive pulmonary diseases

目的观察急性加重期、稳定期慢性阻塞性肺疾病(COPD)患者血中的趋化因子受体3(CXCR3)及淋巴细胞亚群水平,并阐明其对COPD的影响。方法选取徐州医科大学附属医院呼吸内科2015年1月至2016年6月先后收治的急性加重期、稳定期COPD患者以及健康体检人员各43例,将其分为急性加重期组、稳定期组及健康对照组;检测血中T细胞亚群、B淋巴细胞及CXCR3的表达水平,并进行分析比较。结果与健康对照组比较,各病期的COPD患者血中T细胞亚群的CD3+、CD4+、CD4+/CD8+以及B淋巴细胞水平明显降低,差异均有统计学意义(P<0.05),同时,急性加重期组和稳定期组的血中T细胞亚群的趋化因子受体3(CXCR3)在CD4+、CD8+表面的表达均明显增高,且急性加重期组也高于稳定期组,差异有统计学意义(P<0.05)。结论急性加重期的COPD患者机体的细胞免疫系统和体液系统功能失衡,淋巴细胞亚群与其趋化因子受体3对急性加重期的COPD患者的病情发生和发展可能具有重要影响。

Objective To observe the changes in the expression chemokine receptor 3 （CXCR3） and lymphocyte subsets in patients with acute exacerbation and stable chronic obstructive pulmonary disease （COPD） and to elucidate their effect on COPD. Methods During January 2015 to June 2016, 43 cases of acute exacerbating of COPD patients, 43 sta-ble COPD patients, and 43 healthy people in our hospital were selected as the research objects. The expression levels of T lymphocyte subsets, B lymphocytes and CXCR3 in blood were measured and analyzed. Results Compared with the con-trol group, The levels of CD3＋, CD4＋, CD4＋/CD8＋of the T lymphocyte subsets and B lymphocyte subsets were signifi-cantly decreased in different COPD patients （P〈0.05）;while the expression levels of CXCR3 on the surfaces of CD4＋and CD8＋of the T lymphocyte subsets in peripheral blood of different COPD patients were increased significantly, and those of the acute exacerbating of COPD patients were significantly higher than the stable COPD patients （P〈0.05）. Conclusion The functions of the cellular immunity system and the humoral immunity system were dysbalanced appar-ently in patients with COPD acute exacerbating period. Lymphocyte subsets and their CXCR3 may have a significant ef-fect on the onset and development of COPD patients with acute exacerbations.