类番茄茄和多毛番茄CBF1基因转化烟草的表达调控分析

Ectopic expression,regulation and function of CBF1 genes from Solanum lycopersicoides and Lycopersicon habrochaites in tobacco

利用农杆菌介导的叶盘法将类番茄茄(Solanum lycopersicoides)和多毛番茄(Lycopersicon habrochaites)的CBF1基因转入普通烟草(Nicotiana tabacum L.),通过分析转基因烟草在低温胁迫条件下目的基因的表达水平并结合转录组数据分析差异表达的基因,揭示CBF1基因在烟草中发挥抗冷作用的机制与相关性。结果显示:低温胁迫下,转基因烟草的抗低温能力高于非转基因的野生型;SlCBF1和LhCBF1基因在普通烟草中过量表达后,转录组测序分析表明SlCBF1基因转化的烟草植株与野生型烟草植株相比,差异表达基因为53个,其中33个表达上调,20个表达下调;LhCBF1基因转化的烟草和野生型烟草植株相比,差异表达的基因为106个,表达上调的有62个,表达下调的有44个。表明在烟草中过表达SlCBF1和LhCBF1会影响转基因烟草中受其调控的下游基因的表达水平,进而提高烟草的抗冷能力。

The CBF1 gene fromSolanum lycopersicoides and Lycopersicon habrochaites was transferred into tobacco（Nicotiana tobacum L.）by agrobacterium-mediated leaf disc method.The transcriptomic differences of transgenic tobacco under low temperature stress were studied.The results showed that under low temperature stress,the low temperature tolerance of transgenic tobacco was higher than that of the wild type when the transgenic tobacco was under low temperature stress.Both SlCBF1 and LhCBF1 genes were overexpressed in ordinary tobacco.There were 53 differentially expressed genes,33 up-regulated genes and 20 down-regulated genes in transgenic tobacco plants expressing SlCBF1 and wild-type tobacco.There were 106 differentially expressed genes,62 genes up-regulated and 44 downregulated genes in transgenic tobacco plants expressing LhCBF1 gene and wild-type tobacco.It is indicated that SlCBF1 and LhCBF1 may play an important role in transgenic tobacco to obtain anti-low temperature.