PEG胁迫对香石竹幼苗的影响及香石竹抗旱性评价

Effects of PEG stress on carnation seedlings and evaluating drought resistance of different carnation

为探究干旱胁迫对香石竹幼苗的影响,鉴定香石竹各品种的抗旱能力,以9个香石竹品种为研究对象,采用PEG 6000模拟干旱胁迫,分析叶片相对含水量(RWC)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、可溶性蛋白、丙二醛(MDA)、脯氨酸(Pro)等7个生理生化指标的变化,并基于这7个理化指标的抗旱指数,对香石竹各品种进行主成分分析及综合评价。结果表明:9个香石竹品种在水分胁迫处理时各品种各指标存在显著差异(P<0.05)。将7个抗旱指标用主成分分析法归纳成3个主成分,累积贡献率为84.46%,利用隶属函数法评定的抗旱性强弱顺序为马斯特、苔丝、皇族、紫罗兰、火焰、粉恋、锦葵钛合金、粉黛、自由。

The relative water content（RWC）,superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT）,soluble protein,malondialdehyde（MDA）and proline（Pro）under drought stress at 20% PEG 6000 concentration were studied to explore the effect of drought stress on carnation seedlings and evaluate the drought resistance of 9 varieties of carnation.Based on the drought resistance index of these seven physical and chemical indexes,the principal component analysis and comprehensive evaluation of all varieties of carnation were carried out.The results showed that there were significant differences among the indexes of 9 carnation cultivars under water stress（P〈0.05）.Seven drought resistance indexes were classified into four principal components by principal component analysis,with the cumulative contribution rate of 84.46%.The drought resistance evaluated by the membership function method is in the order of‘Master＇,‘Tess＇,‘Royal＇,‘Violet＇,‘Flame＇,‘Pink Love＇,‘Mallow Titanium Alloy＇,‘Pink Superior＇,‘Freedom＇.