湖北省蔬菜病毒病主要毒原种类检测

Detection of main viral species of vegetable virus diseases in Hubei Province

采用斑点酶联免疫吸附法(Dot-ELISA)和RT-PCR法对采自湖北省7个地区的966份疑似感染病毒的蔬菜样本进行黄瓜花叶病毒(Cucumber mosaic virus,CMV)、烟草花叶病毒(Tobacco mosaic virus,TMV)、番茄斑萎病毒(Tomato spotted wilt virus,TSWV)、番茄花叶病毒(Tomato mosaic virus,ToMV)、芜菁花叶病毒(Turnip mosaic virus,TuMV)、蚕豆萎蔫病毒(Broad bean wilt virus,BBWV)、黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)共7种病毒的检测。结果显示检出阳性样本695份,占总样本数的71.9%。其中,十字花科蔬菜上主要毒原为TuMV,检出率高达86.4%,其次为CMV,检出率为26.7%;茄科、葫芦科和豆科蔬菜上主要毒原均为CMV,检出率分别为34.3%、59.2%和56.2%。CMV几乎能侵染所有蔬菜,为湖北省蔬菜病毒病的主要毒原。在检出的6种病毒中,存在TuMV+CMV、TuMV+BBWV、TuMV+CMV+BBWV、CMV+BBWV、CMV+ToMV和CMV+BBWV+TSWV+TMV等6种复合侵染类型,其中TuMV+CMV为十字花科蔬菜上的主要复合侵染类型,复合侵染率为23.8%,其他蔬菜上主要侵染类型为CMV+BBWV,复合侵染率为28.0%。

Nine hundred and sixty-six suspected vegetable samples were collected from seven regions in Hubei Province.Seven viruses including Cucumber mosaic virus（CMV）,Tobacco mosaic virus（TMV）,Tomato spotted wilt virus（TSWV）,Tomato mosaic virus（ToMV）,Turnip mosaic virus（TuMV）,Broad bean wilt virus（BBWV）and Cucumber green mottle mosaic virus（CGMMV）were detected by Dot-ELISA and RT-PCR.The results showed that 695 of the 966 samples were positive for virus infection,accounting for an incidence of 71.9%.Of which,TuMV ranked the highest with an incidence of 86.4%,followed by CMV with an incidence of 26.7%,in brassicaceous vegetables.CMV infected solanaceous,cucurbitaceous and leguminous vegetables with an incidence of 34.3%,59.2% and 56.2%,respectively.CMV,which infected almost all vegetable plants,was the major virus infecting vegetables in Hubei province.For the six viruses detected,they infected plants in six mixed patterns,including TuMV＋CMV,TuMV＋BBWV,TuMV＋CMV＋BBWV,CMV＋BBWV,CMV＋ToMV and CMV＋BBWV＋TSWV＋TMV.Of which,TuMV＋CMV and CMV＋BBWV were the major combination of the mixed infection in brassicaceous vegetables and the other vegetables,accounting for an incidence of23.8% and 28.0%,respectively.