hexarelin预处理对小鼠肾脏缺血/再灌注损伤的保护作用

Hexarelin Pretreatment Protects against Renal Ischemia/reperfusion Injury in Mice

目的研究生长激素释放肽(GHRP)家族成员hexarelin是否对肾缺血-再灌注损伤具有保护作用及其可能机制。方法在体情况下结扎C57BL6/J小鼠左侧肾动脉主干2h、松扎0.5h造成急性全肾缺血/再灌注损伤。hexarelin预处理组于结扎肾动脉前2h给予小鼠皮下注射hexarelin(100μg/kg);单纯肾缺血/再灌注组用生理盐水替代hexarelin;正常对照组仅给予假手术而不结扎肾动脉。肾组织切片TTC染色鉴定肾缺血/再灌注损伤面积。苏木精-伊红染色观察肾组织微观损伤情况。测定肾组织丙二醛(MDA)及总抗氧化能力(T-AOC)含量以判断氧化应激情况。结果与单纯肾缺血/再灌注组相比,hexarelin预处理使肾缺血/再灌注损伤面积平均减小约49%(P<0.01);同时,肾缺血/再灌注导致肾组织丙二醛水平升高及总抗氧化能力降低,而hexarelin预处理则可明显改善由缺血/再灌注导致的MDA升高和T-AOC降低。结论 hexarelin对肾缺血/再灌注损伤具有明显的保护作用,其可能机制可能与降低肾组织氧化应激水平并提高总抗氧化能力有关。

Objective To investigate whether hexarelin, a kind of growth hormone releasing peptides, could protect against renal is- chemia/reperfusion （I/R） injury. Methods Renal I/R injury was created by ligating the left renal artery for 2h and then releasing the ligation for 0.5h. Hexarelin （ 100ttg/kg） was subcutaneously injected 2h before renal artery ligation. Normal control mice subjected to sham surgery and saline injection. The area of renal I/R injury was determined by TTC staining. Renal oxidative stress and total antioxi- dant capacity （T - AOC） were measured by kits for malondialdehyde （MDA） and T - AOC assays. Results Compared with the renal I/ R group, hexarelin pretreatment significantly reduced the area of renal I/R injury by 49% （P 〈 0. O1 ）. In addition, renal I/R injury sig- nificantly increased the renal MDA level but decreased the T - AOC, while hexarelin attenuated the increase of MDA and decrease of T - AOC induced by renal I/R. Conclusion Hexarelin significantly protects against renal I/R injury potentially via decreasing renal oxida- tive stress and increasing the total antioxidant capacity.