黄芪甲苷对苯并芘诱导RAW264.7细胞MMPs表达的保护作用

Benzopyrene Induced the Expression of MMPs and the Effects of Astragaloside in RAW264.7 Cells

目的观察黄芪甲苷(astragaloside IV,ASIV)对苯并芘(benzopyrene,Bap)诱导RAW264.7巨噬细胞MMPs表达的保护作用,探讨腹主动脉瘤防治的可能新策略。方法应用Western blot法观察Bap对RAW264.7巨噬细胞基质金属蛋白酶-9/12(MMP-9、MMP-12)、NF-κB、TNF-α等表达的变化及黄芪甲苷的作用,利用流式细胞仪观察黄芪甲苷对巨噬细胞ROS表达的影响。结果 Bap能够诱导RAW264.7巨噬细胞MMP-9、MMP-12等金属蛋白酶的表达升高和NF-κB、TNF-α等炎性指标的活化。黄芪甲苷具有降低RAW264.7巨噬细胞因Bap诱导而表达升高的MMP-9、MMP-12、NF-κB、TNF-α,同时具有清除ROS的作用。结论黄芪甲苷能够降低Bap刺激RAW264.7巨噬细胞引起的MMP-9、MMP-12、NF-κB、TNF-α的表达升高,并具有清除ROS的作用。

Objective To explore the effect of astragaloside IV （ASIV） in Bap induced the expression of MMPs on RAW264.7 cells, to provide a new control strategy of abdominal aortic aneurysm caused by smoking. Methods We observed the influence of ASIV on matrix metalloproteinase - 9/12 （ MMP - 9, MMP - 12） and NF - kB, TNF - α of RAW264.7 cells in vitro by western blotting. We used flow cytometry instrument to analysis whether ASIV has the role of reducing ROS. Results Bap caused expression of MMP - 9, MMP - 12, NF - KB, TNF - α increased in cultured RAW264.7 cells. ASIV has the effect of decreasing the expression of MMP - 9, MMP - 12, NF - KB, TNF -α, otherwise it can eliminate the ROS which was caused by Bap incultured RAW264.7 ceils. Conclusion ASIV can decrease the expression of MMP -9, MMP - 12, NF - KB,TNF - α and eliminate the ROS in RAW264. 7 cells cultured with Bap.