安徽省克氏原螯虾白斑综合征病毒变异区序列分析

Sequence analysis of variable regions of white spot syndrome virus from Procambarus clarkii in Anhui Province

白斑综合征已给安徽省克氏原螯虾产业带来了巨大的经济损失。为了解安徽省白斑综合征病毒(white spot syndrome virus,WSSV)分子流行病学,2016年4—8月在安徽省6个市采集9个养殖的克氏原螯虾样本进行WSSV套式PCR检测,扩增病毒可变区ORF94、ORF75和ORF125,将获得序列进行比较分析。结果显示,9个样本均在第1轮PCR扩增中获得阳性结果,其ORF94的重复单元(repeat unit,RU)数量为2、4、7、8和12,2RUs为常见基因型,ORF75的组合RUs总数为3、6、9、10和11,9 RUs为常见基因型,排列顺序为45、102、45、102、3×45、102和45,ORF125的RUs为5~9,其中7 RUs和6 RUs为常见基因型。

White spot syndrome has caused significant economic loss in Procambarus clarkii industry in Anhui Province. To understand the molecular epidemiology of white spot syndrome virus （WSSV）, we collected nine cultured P clarkii samples between April and August 2016 from six cities in Anhui Province and performed nested-PCR analyses to detect WSSV and amplify the sequences of ORF94, ORF75 and ORF 125 variable regions. The results were as follows： nine samples were all WSSV positive in the first PCR amplification. The numbers of repeats units （RU） in ORF94 were 2, 4, 7, 8 and 12, while 2 RUs was the dominant genotype. The numbers of compound RUs in ORF75 were 3, 6, 9, 10 and 11. The most dominant genotype displayed 9 RUs with the posi- tioning of 45, 102, 45, 102, 3×45, 102 and 45. The RUs numbers of ORF125 ranged from 5 to 9, and the most prevalent genotypes were 7 RUs and 6 RUs.