通精灵对实验性精索静脉曲张大鼠精子DNA完整性与睾丸组织氧化应激的影响

Tongjingling improves sperm DNA integrity and reduces oxidative stress in the testis of experimental varicocele rats

目的:探讨通精灵对实验性精索精索静脉曲张大鼠精子DNA完整性与睾丸组织氧化应激的影响。方法:雄性Wistar大鼠75只随机分为假手术组、模型组、高剂量组、中剂量组、低剂量组,每组15只。参照文献制备大鼠精索静脉曲张模型,"夹尾激怒法"建立合并中医肝气郁结证模型。造模完成后4周开始给药,持续8周。观察大鼠的一般情况,精子染色质结构分析法(SCSA)检测附睾精子DFI。化学比色法测睾丸过氧化氢(H_2O_2)含量、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性。结果:与假手术组相比较,模型组大鼠附睾精子DFI[(8.36±2.49)%]显著升高(P<0.01),睾丸组织H_2O_2含量[(431.22±97.01)mmol/g prot]显著升高(P<0.01),CAT[(10.53±2.69)U/ml]、SOD[(87.30±25.33)U/ml]活性显著升高(P<0.01)。与模型组相比较,通精灵各组附睾精子DFI显著降低,睾丸组织H_2O_2的含量显著降低。结论:精索静脉曲张模型大鼠附睾精子DNA完整性降低,睾丸组织呈氧化应激状态,精子DNA完整性与氧化应激有相关性,通精灵可能通过增加CAT、SOD活性,降低H_2O_2含量减轻了睾丸组织氧化应激状态,从而提高精子DNA完整性。

Objective： To explore the protective effect of Tongjingling （TJL） against sperm DNA damage and oxidative stress in the rat model of experimental varicocele （EVC）. Methods ： We randomly divided 75 Wistar male rats into five groups of equal number： sham operation, EVC model, high-dose TJL, mid-dose TJL, and low-dose TJL. The EVC model was established in the rats by partial ligation of the left renal vein, followed by 8 weeks of medication from the 4th week after modeling. Then we observed the general status of the rats, detected the sperm DNA fragmentation index （DFI） in the epididymis by sperm chromatin structure assay （SCSA）, and measured the content of hydroperoxide （H202 ） and the activities of catalase （CAT） and superoxide dismutase （SOD） in the testis by colorimetry. Results： Compared with the sham operation group, the EVC models showed significantly increased sperm DFI in the epididymis （P 〈0.01 ） and elevated level of H202 and activities of CAT and SOD in the testis （P 〈0.01 ）. In comparison with the EVC models, the rats of the TJL groups exhibited remarkably reduced sperm DFI and H20z content, but increased activities of SOD and CAT. Conclusion： TJL can improve sperm DNA integrity by increasing the activities of SOD and CAT and reducing the H202 level and hence oxidative stress in the testis tissue.