复方芪芎颗粒治疗类风湿性关节炎的机制

Mechanism of Compound Qixiong Particles in the treatment of rheumatoid arthritis

目的:探索复方芪芎颗粒治疗类风湿性关节炎的作用机制。方法:随机将60只Wistar大鼠分成空白组、模型组、复方芪芎颗粒高剂量组(高剂量组)、复方芪芎颗粒中剂量组(中剂量组)、复方芪芎颗粒低剂量组(低剂量组)、雷公藤组。除空白组外其余大鼠通过弗氏完全佐剂诱导建立佐剂型关节炎模型。造模2周后,进行给药。空白组和模型组每日给予纯化水10 mL·kg^(-1),高、中、低剂量组分别给予0.05,0.045,0.04 g·mL-1复方芪芎颗粒水溶液10 mL·kg^(-1)、雷公藤组给予0.94 mg·mL-1雷公藤多苷片水溶液10 mL·kg^(-1),给药每日1次,持续3周。主要检测指标包括:大鼠足爪的肿胀度、大鼠TLR2、TLR4、TLR9的蛋白和mRNA的表达水平。结果:与空白组比较,造模大鼠均表现出足爪肿胀增高、TLR2、TLR4、TLR9蛋白与mRNA水平的升高(P<0.01)。与模型组比较,给药组均能不同程度抑制大鼠足爪的肿胀和TLR2、TLR4、TLR9蛋白和mRNA的表达水平(P<0.05)。其中高剂量组与中剂量组的抑制作用优于小剂量组与雷公藤组(P<0.05)。结论:复方芪芎颗粒能抑制佐剂型关节炎大鼠的关节炎症表现,这种抑制作用优于雷公藤多苷片,其作用机制可能与抑制TLRs的过度表达有关。

OBJECTIVE To explore the action mechanism of Compound Qixiong Particles（CQXP）in the treatment of rheumatoid arthritis.METHODS Sixty Wistar rats were randomly divided into control group,model group,Tripterygium Glycosides tablets（TGT）group,CQXP high dose group（CQXP-H）,CQXP middle dose group（CQXP-M）and CQXP low dose group（CQXP-L）.Except for the control group,rats of other groups were induced by complete Freund＇s adjuvant（CFA）to establish adjuvant arthritis（AA）rat models.Two weeks after the first modeling,rats in each drug groups were given medicines accordingly by gastric gavage once a day for 3 weeks.Rats in the control group and model group were given isopyknic normal saline in the same way.Swelling dimension of joints and the protein and mRNA expression levels of TLR-2,TLR-4 and TLR-9 were main outcome measures.RESULTS Three weeks after treatment,the expression levels of these factors were significantly down regulated in the drug treated groups compared with model group（P0.05）.The therapeutic effects of CQXP-H group and CQXP-M group were obviously better than the effect of CQXP-L group and TGT group（P0.05）.CONCLUSION These results indicate that CQXP exerts therapeutic effects on the AA rat model,and its therapeutic mechanism may be associated with its inhibition of TLRs excessive expression.