血液恶性肿瘤患者肠道菌群分析

Analysis of gut microbiota from patients with hematologic malignancy

目的将血液恶性肿瘤患者肠道菌群与健康个体进行比较,观察血液肿瘤患者肠道菌群结构的变化,探讨肠道菌群与血液恶性肿瘤发生发展的联系。方法收集血液恶性肿瘤患者与健康志愿者粪便样品,提取样品中菌群总DNA,然后通过变性凝胶梯度电泳(DGGE)技术分析肠道菌群多样性和差异性。结果血液恶性肿瘤组与健康组肠道菌群的DGGE指纹图谱有明显差异。与正常组相比,患者组肠道大肠埃希菌呈现过度增长趋势,有益菌柔嫩梭菌减少或缺失,某些患者肠道内一些细菌呈现特异性增长,如粪肠球菌、硫磺肠球菌、约氏不动杆菌等。结论与健康对照组相比,血液恶性肿瘤患者肠道菌群结构与多样性发生改变,这可能为血液恶性肿瘤早期抗感染提供实验依据。

Objective To observe the connection between hematologic malignancy and gut microbiota by comparing gut microbiota between hematologic malignancy patients and healthy individuals.Methods Fecal samples were collected from the patients and healthy volunteers.The total DNA of fecal bacteria was extracted.PCR-DGGE was used to detect the diversity of intestinal flora in each group.Results There was a significant difference in DGGE fingerprint between the two groups.Comparing with the healthy individuals,the patient group showed an overgrowth of E.coli and reduction or missing of Faecalibacterium prausnitzii.In some patients,some of intestinal bacteria such as Enterococcus faecalis,Enterococcus sulfureus and Acinetobacter johnsonii increased specifically.Conclusion Significant changes in gut microbiota diversity was observed in patients with hematologic malignancy,which may help in early anti-infection of hematologic malignancy.