采用纳米金修饰的方法再生碳纤维微电极

Modification with gold nanoparticle-a new method for the regeneration of carbon fiber microelectrodes

采用电沉积法将纳米金(GNP)修饰到毒化的碳纤维电极表面制得再生碳纤维电极,用循环伏安法(CV)和差分脉冲伏安法(DPV)对神经递质在该再生电极上的电化学行为进行了研究。实验结果表明,在20 mmol/L,pH7.2的Tris-HCl缓冲溶液中,再生电极对多巴胺、去甲肾上腺素等神经递质具有良好的电化学响应。对纳米金的电沉积时间进行了优化,在优化条件下,采用DPV法,DA的氧化峰电流与其浓度在5×10^(-7)~1×10^(-4)mol/L范围内呈良好的线性关系,线性相关系数(R^2)为0.9979,检出限为5×10^(-7)mol/L。

In this work, the fouling carbon fiber microelectrodes was regenerated by modification with novel gold nanoparticles（GNP） using electrodeposition method, and the electrochemical behaviors of neurotransmitters solution on the regenerated electrode was investigated by cyclic voltammetry （CV） and different pulse voltammetry （DPV）. The electrochemical experimental results showed that in pH7.2, 20 mmol/L Tris-HC1 buffer solution, the regenerated electrode showed excellent electrochemical response to dopamine （DA） and norepinephrine （NE）. Optimization experiment was also conducted for the GNP electrodeposition time. Under the optimized condition, there was a good linear relationship between oxidation peak current and DA concentration, in the range of 5 ×10^-7 1 × 10^-4mol/L, with a detection limit of 5×lO^-7moL/L.