石墨烯修饰电化学适配体传感器测定双酚A

Electrochemical aptamer sensor based on graphene modified glassy carbon electrode for the label-free detection of bisphenol A

构建了一种检测双酚A(BPA)的电化学适配体传感器。利用在线电化学方法将氧化石墨烯还原为石墨烯,通过石墨烯与单链DNA之间的相互作用,将BPA适配体单链DNA吸附固定在修饰电极上,制备了BPA电化学适配体传感器。以铁氰化钾-亚铁氰化钾平衡电对为电化学探针,利用电化学循环伏安法和差分脉冲伏安法对BPA传感器的性能进行了研究。结果表明,在最优化实验条件下,传感器对BPA的检测线性范围在1.0×10^(-15)~1.0×10^(-10)mol/L之间,检出限为3.3×10^(-16)mol/L(S/N=3)。

A simple, fast and sensitive electrochemical aptamer sensor for the determination of biaphenol A （BPA） was constructed based on graphene modified glassy carbon electrode. Graphene oxide was reduced to graphene on line by electrochemical method. The BPA aptamer DNA was immobilized on the graphene modified electrode through the interaction with graphene to prepare the BPA electrochemical aptamer sensor. With ferricyanide - ferrocyanide （ 1 ： 1 ） as the electrochemical probe, the performance of BPA aptamer sensor was investigated by cyclic voltammetry （CV） and differential pulse vohammetry （DPV）. The results showed that under the optimization of experimental conditions, the peak currents linearly correlated with the BPA concentration over a linear range of 1.0 × 10^-15- 1.0×10^-10 mol/L, with a detection limit of 3.3 ×10^-16 mol/L（ S/N = 3 ）. Featured by the wide linear range and low detection limit, the electrochemical aptamer sensor based on graphene modified electrode can be used for the detection of other environmental hormone.